{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:58:31Z","timestamp":1791665911617,"version":"4.3.4"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Adenosine, a purine nucleoside, is present at high concentrations in tumors, where it contributes to the failure of immune cells to eliminate cancer cells. The mechanisms responsible for the immunosuppressive properties of adenosine are not fully understood. We tested the hypothesis that adenosine\u2019s immunosuppressive functions in human T lymphocytes are in part mediated via modulation of ion channels. The activity of T lymphocytes relies on ion channels. KCa3.1 and Kv1.3 channels control cytokine release and, together with TRPM7, regulate T cell motility. Adenosine selectively inhibited KCa3.1, but not Kv1.3 and TRPM7, in activated human T cells. This effect of adenosine was mainly mediated by A2A receptors, as KCa3.1 inhibition was reversed by SCH58261 (selective A2A receptor antagonist), but not by MRS1754 (A2B receptor antagonist), and it was mimicked by the A2A receptor agonist CGS21680. Furthermore, it was mediated by the cAMP\/protein kinase A isoform (PKAI) signaling pathway, as adenylyl-cyclase and PKAI inhibition prevented adenosine effect on KCa3.1. The functional implication of the effect of adenosine on KCa3.1 was determined by measuring T cell motility on ICAM-1 surfaces. Adenosine and CGS21680 inhibited T cell migration. Comparable effects were obtained by KCa3.1 blockade with TRAM-34. Furthermore, the effect of adenosine on cell migration was abolished by pre-exposure to TRAM-34. Additionally, adenosine suppresses IL-2 secretion via KCa3.1 inhibition. Our data indicate that adenosine inhibits KCa3.1 in human T cells via A2A receptor and PKAI, thereby resulting in decreased T cell motility and cytokine release. This mechanism is likely to contribute to decreased immune surveillance in solid tumors.<\/jats:p>","DOI":"10.4049\/jimmunol.1300702","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T05:42:58Z","timestamp":1384407778000},"page":"6273-6280","source":"Crossref","is-referenced-by-count":54,"title":["Selective Inhibition of KCa3.1 Channels Mediates Adenosine Regulation of the Motility of Human T Cells"],"prefix":"10.1093","volume":"191","author":[{"given":"Ameet A","family":"Chimote","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hajdu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Kucher","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Boiko","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zerrin","family":"Kuras","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orsolya","family":"Szilagyi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeo-Heung","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, North Carolina Agricultural and Technical State University , Greensboro, NC 27411"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Conforti","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,12,15]]},"reference":[{"key":"2025032406044125600_r1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1046\/j.1471-4159.2000.740621.x","article-title":"Chronic hypoxia enhances adenosine release in rat PC12 cells by altering adenosine metabolism and membrane transport","volume":"74","author":"Kobayashi","year":"2000","journal-title":"J. Neurochem."},{"key":"2025032406044125600_r2","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1146\/annurev.immunol.22.012703.104731","article-title":"Physiological control of immune response and inflammatory tissue damage by hypoxia-inducible factors and adenosine A2A receptors","volume":"22","author":"Sitkovsky","year":"2004","journal-title":"Annu. Rev. Immunol."},{"key":"2025032406044125600_r3","doi-asserted-by":"crossref","first-page":"S272","DOI":"10.1016\/j.jaci.2009.09.045","article-title":"Immune responses to malignancies","volume":"125","author":"Whiteside","year":"2010","journal-title":"J. Allergy Clin. Immunol."},{"key":"2025032406044125600_r4","first-page":"2602","article-title":"The extracellular fluid of solid carcinomas contains immunosuppressive concentrations of adenosine","volume":"57","author":"Blay","year":"1997","journal-title":"Cancer Res."},{"key":"2025032406044125600_r5","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.it.2008.12.002","article-title":"T regulatory cells: hypoxia-adenosinergic suppression and re-direction of the immune response","volume":"30","author":"Sitkovsky","year":"2009","journal-title":"Trends Immunol."},{"key":"2025032406044125600_r6","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S1096-4959(00)00326-2","article-title":"The molecular basis of O2-sensing and hypoxia tolerance in pheochromocytoma cells","volume":"128","author":"Conrad","year":"2001","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"2025032406044125600_r7","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1084\/jem.20062512","article-title":"Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression","volume":"204","author":"Deaglio","year":"2007","journal-title":"J. Exp. Med."},{"key":"2025032406044125600_r8","doi-asserted-by":"crossref","first-page":"7176","DOI":"10.1074\/jbc.M109.047423","article-title":"Generation and accumulation of immunosuppressive adenosine by human CD4+CD25highFOXP3+ regulatory T cells","volume":"285","author":"Mandapathil","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2025032406044125600_r9","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1161\/ATVBAHA.111.226837","article-title":"Regulation of lymphocyte function by adenosine","volume":"32","author":"Linden","year":"2012","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2025032406044125600_r10","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1056\/NEJMra1205750","article-title":"Purinergic signaling during inflammation","volume":"367","author":"Eltzschig","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"2025032406044125600_r11","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0031-6997(24)01511-4","article-title":"International Union of Pharmacology. XXV. Nomenclature and classification of adenosine receptors","volume":"53","author":"Fredholm","year":"2001","journal-title":"Pharmacol. Rev."},{"key":"2025032406044125600_r12","doi-asserted-by":"crossref","first-page":"5346","DOI":"10.1038\/onc.2010.292","article-title":"Extracellular adenosine triphosphate and adenosine in cancer","volume":"29","author":"Stagg","year":"2010","journal-title":"Oncogene"},{"key":"2025032406044125600_r13","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.4049\/jimmunol.174.2.1073","article-title":"A2A adenosine receptor induction inhibits IFN-gamma production in murine CD4+ T cells","volume":"174","author":"Lappas","year":"2005","journal-title":"J. Immunol."},{"key":"2025032406044125600_r14","doi-asserted-by":"crossref","first-page":"5487","DOI":"10.4049\/jimmunol.0901247","article-title":"A2A adenosine receptor may allow expansion of T cells lacking effector functions in extracellular adenosine-rich microenvironments","volume":"183","author":"Ohta","year":"2009","journal-title":"J. Immunol."},{"key":"2025032406044125600_r15","doi-asserted-by":"crossref","first-page":"13132","DOI":"10.1073\/pnas.0605251103","article-title":"A2A adenosine receptor protects tumors from antitumor T cells","volume":"103","author":"Ohta","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032406044125600_r16","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1096\/fj.11-197046","article-title":"Distinctive immunoregulatory effects of adenosine on T cells of older humans","volume":"26","author":"Hesdorffer","year":"2012","journal-title":"FASEB J."},{"key":"2025032406044125600_r17","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1002\/eji.200637024","article-title":"Adenosine closes the K+ channel KCa3.1 in human lung mast cells and inhibits their migration via the adenosine A2A receptor","volume":"37","author":"Duffy","year":"2007","journal-title":"Eur. J. Immunol."},{"key":"2025032406044125600_r18","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1152\/physrev.00018.2011","article-title":"Role of ion channels and transporters in cell migration","volume":"92","author":"Schwab","year":"2012","journal-title":"Physiol. Rev."},{"key":"2025032406044125600_r19","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1600-065X.2009.00816.x","article-title":"The functional network of ion channels in T lymphocytes","volume":"231","author":"Cahalan","year":"2009","journal-title":"Immunol. Rev."},{"key":"2025032406044125600_r20","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1038\/nri3233","article-title":"Ion channels and transporters in lymphocyte function and immunity","volume":"12","author":"Feske","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"2025032406044125600_r21","first-page":"212","article-title":"Potassium channels keep mobile cells on the go","volume":"23","author":"Schwab","year":"2008","journal-title":"Physiology (Bethesda)"},{"key":"2025032406044125600_r22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2776(08)00001-1","article-title":"T cell activation and the cytoskeleton: you can\u2019t have one without the other","volume":"97","author":"Gomez","year":"2008","journal-title":"Adv. Immunol."},{"key":"2025032406044125600_r23","doi-asserted-by":"crossref","first-page":"e43859","DOI":"10.1371\/journal.pone.0043859","article-title":"KCa3.1 and TRPM7 channels at the uropod regulate migration of activated human T cells","volume":"7","author":"Kuras","year":"2012","journal-title":"PLoS ONE"},{"key":"2025032406044125600_r24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ceca.2009.11.001","article-title":"Differential calcium signaling and Kv1.3 trafficking to the immunological synapse in systemic lupus erythematosus","volume":"47","author":"Nicolaou","year":"2010","journal-title":"Cell Calcium"},{"key":"2025032406044125600_r25","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1113\/jphysiol.2006.109967","article-title":"Signalling during hypoxia in human T lymphocytes\u2014critical role of the src protein tyrosine kinase p56Lck in the O2 sensitivity of Kv1.3 channels","volume":"573","author":"Szigligeti","year":"2006","journal-title":"J. Physiol."},{"key":"2025032406044125600_r26","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1113\/jphysiol.2004.081893","article-title":"Hypoxia modulates early events in T cell receptor-mediated activation in human T lymphocytes via Kv1.3 channels","volume":"564","author":"Robbins","year":"2005","journal-title":"J. Physiol."},{"key":"2025032406044125600_r27","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1242\/jcs.00606","article-title":"LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment","volume":"116","author":"Smith","year":"2003","journal-title":"J. Cell Sci."},{"key":"2025032406044125600_r28","doi-asserted-by":"crossref","first-page":"8151","DOI":"10.1073\/pnas.97.14.8151","article-title":"Design of a potent and selective inhibitor of the intermediate-conductance Ca2+-activated K+ channel, IKCa1: a potential immunosuppressant","volume":"97","author":"Wulff","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032406044125600_r29","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1023\/A:1010958907271","article-title":"Molecular properties and physiological roles of ion channels in the immune system","volume":"21","author":"Cahalan","year":"2001","journal-title":"J. Clin. Immunol."},{"key":"2025032406044125600_r30","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/S0022-3565(25)12287-8","article-title":"The non-xanthine heterocyclic compound SCH 58261 is a new potent and selective A2a adenosine receptor antagonist","volume":"276","author":"Zocchi","year":"1996","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"2025032406044125600_r31","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/S0006-2952(01)00531-7","article-title":"[3H]MRS 1754, a selective antagonist radioligand for A(2B) adenosine receptors","volume":"61","author":"Ji","year":"2001","journal-title":"Biochem. Pharmacol."},{"key":"2025032406044125600_r32","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1152\/japplphysiol.00459.2005","article-title":"Adenosine A2A receptors mediate GABAergic inhibition of respiration in immature rats","volume":"100","author":"Mayer","year":"2006","journal-title":"J. Appl. Physiol."},{"key":"2025032406044125600_r33","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/nrd1983","article-title":"Adenosine receptors as therapeutic targets","volume":"5","author":"Jacobson","year":"2006","journal-title":"Nat. Rev. Drug Discov."},{"key":"2025032406044125600_r34","doi-asserted-by":"crossref","first-page":"5159","DOI":"10.4049\/jimmunol.179.8.5159","article-title":"Inhibition of T cell activation by cyclic adenosine 5\u2032-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin","volume":"179","author":"Ruppelt","year":"2007","journal-title":"J. Immunol."},{"key":"2025032406044125600_r35","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1074\/jbc.271.5.2380","article-title":"2\u2032,5\u2032-Dideoxyadenosine 3\u2032-polyphosphates are potent inhibitors of adenylyl cyclases","volume":"271","author":"D\u00e9saubry","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2025032406044125600_r36","doi-asserted-by":"crossref","first-page":"C1504","DOI":"10.1152\/ajpcell.00263.2011","article-title":"Modulation of Kv1.3 channels by protein kinase A I in T lymphocytes is mediated by the disc large 1-tyrosine kinase Lck complex","volume":"302","author":"Kuras","year":"2012","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2025032406044125600_r37","doi-asserted-by":"crossref","first-page":"3431","DOI":"10.1128\/IAI.05005-11","article-title":"Activation of adenosine A2A receptors inhibits neutrophil transuroepithelial migration","volume":"79","author":"S\u00e4ve","year":"2011","journal-title":"Infect. Immun."},{"key":"2025032406044125600_r38","doi-asserted-by":"crossref","first-page":"C1592","DOI":"10.1152\/ajpcell.00546.2005","article-title":"Adenosine stimulates depolarization and rise in cytoplasmic [Ca2+] in type I cells of rat carotid bodies","volume":"290","author":"Xu","year":"2006","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2025032406044125600_r39","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1469-7793.1998.095br.x","article-title":"Adenosine modulates hypoxia-induced responses in rat PC12 cells via the A2A receptor","volume":"508","author":"Kobayashi","year":"1998","journal-title":"J. Physiol."},{"key":"2025032406044125600_r40","doi-asserted-by":"crossref","first-page":"C1443","DOI":"10.1152\/ajpcell.00556.2007","article-title":"Apical adenosine regulates basolateral Ca2+-activated potassium channels in human airway Calu-3 epithelial cells","volume":"294","author":"Wang","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2025032406044125600_r41","doi-asserted-by":"crossref","first-page":"10963","DOI":"10.1074\/jbc.M007716200","article-title":"ATP-dependent activation of the intermediate conductance, Ca2+-activated K+ channel, hIK1, is conferred by a C-terminal domain","volume":"276","author":"Gerlach","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2025032406044125600_r42","doi-asserted-by":"crossref","first-page":"C635","DOI":"10.1152\/ajpcell.00283.2003","article-title":"ATP-dependent regulation of SK4\/IK1-like currents in rat submandibular acinar cells: possible role of cAMP-dependent protein kinase","volume":"286","author":"Hayashi","year":"2004","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2025032406044125600_r43","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1007\/s004240050698","article-title":"Modulation of Ca2+-activated K+ channels of human erythrocytes by endogenous cAMP-dependent protein kinase","volume":"436","author":"Pellegrino","year":"1998","journal-title":"Pflugers Arch."},{"key":"2025032406044125600_r44","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1007\/s00424-004-1302-5","article-title":"Protein kinase A inhibits intermediate conductance Ca2+-activated K+ channels expressed in Xenopus oocytes","volume":"448","author":"Neylon","year":"2004","journal-title":"Pflugers Arch."},{"key":"2025032406044125600_r45","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1084\/jem.191.7.1167","article-title":"Extracellular K(+) and opening of voltage-gated potassium channels activate T cell integrin function: physical and functional association between Kv1.3 channels and beta1 integrins","volume":"191","author":"Levite","year":"2000","journal-title":"J. Exp. Med."},{"key":"2025032406044125600_r46","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.immuni.2008.07.015","article-title":"Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block","volume":"29","author":"Matheu","year":"2008","journal-title":"Immunity"},{"key":"2025032406044125600_r47","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1172\/JCI30836","article-title":"The intermediate-conductance calcium-activated potassium channel KCa3.1 contributes to atherogenesis in mice and humans","volume":"118","author":"Toyama","year":"2008","journal-title":"J. Clin. Invest."},{"key":"2025032406044125600_r48","doi-asserted-by":"crossref","first-page":"37137","DOI":"10.1074\/jbc.M003941200","article-title":"Up-regulation of the IKCa1 potassium channel during T-cell activation. Molecular mechanism and functional consequences","volume":"275","author":"Ghanshani","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2025032406044125600_r49","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1038\/341619a0","article-title":"T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1","volume":"341","author":"Dustin","year":"1989","journal-title":"Nature"},{"key":"2025032406044125600_r50","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1096\/fj.08-107458","article-title":"Adenosine A2A receptor activation inhibits T helper 1 and T helper 2 cell development and effector function","volume":"22","author":"Cs\u00f3ka","year":"2008","journal-title":"FASEB J."},{"key":"2025032406044125600_r51","doi-asserted-by":"crossref","first-page":"659","DOI":"10.4161\/onci.20387","article-title":"CD26 expression and adenosine deaminase activity in regulatory T cells (Treg) and CD4(+) T effector cells in patients with head and neck squamous cell carcinoma","volume":"1","author":"Mandapathil","year":"2012","journal-title":"OncoImmunology"},{"key":"2025032406044125600_r52","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1158\/0008-5472.CAN-11-3218","article-title":"Immunotype and immunohistologic characteristics of tumor-infiltrating immune cells are associated with clinical outcome in metastatic melanoma","volume":"72","author":"Erdag","year":"2012","journal-title":"Cancer Res."},{"key":"2025032406044125600_r53","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1084\/jem.20060710","article-title":"Random migration precedes stable target cell interactions of tumor-infiltrating T cells","volume":"203","author":"Mrass","year":"2006","journal-title":"J. Exp. Med."},{"key":"2025032406044125600_r54","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1074\/jbc.M111.274209","article-title":"Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes","volume":"287","author":"Chimote","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2025032406044125600_r55","doi-asserted-by":"crossref","first-page":"695","DOI":"10.4049\/jimmunol.170.2.695","article-title":"Hypoxia regulates expression and activity of Kv1.3 channels in T lymphocytes: a possible role in T cell proliferation","volume":"170","author":"Conforti","year":"2003","journal-title":"J. Immunol."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/191\/12\/6273\/61535372\/1300702.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/191\/12\/6273\/61535372\/1300702.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T16:13:18Z","timestamp":1742919198000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/191\/12\/6273\/7966427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":55,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1300702","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2013,12]]},"published":{"date-parts":[[2013,12]]}}}