{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T10:25:45Z","timestamp":1774952745786,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dendritic cells (DCs) are potent APCs essential for initiating adaptive immunity. Following pathogen exposure, trafficking of DCs to lymph nodes (LNs) through afferent lymphatic vessels constitutes a crucial step in the execution of their functions. The mechanisms regulating this process are poorly understood, although the involvement of certain chemokines in this process has recently been reported. In this study, we demonstrate that genetically altering the fine structure (N-sulfation) of heparan sulfate (HS) specifically in mouse lymphatic endothelium significantly reduces DC trafficking to regional LNs in vivo. Moreover, this alteration had the unique functional consequence of reducing CD8+ T cell proliferative responses in draining LNs in an ovalbumin immunization model. Mechanistic studies suggested that lymphatic endothelial HS regulates multiple steps during DC trafficking, including optimal presentation of chemokines on the surface of DCs, thus acting as a co-receptor that may function \u201cin trans\u201d to mediate chemokine receptor binding. This study not only identifies novel glycan-mediated mechanisms that regulate lymphatic DC trafficking, but it also validates the fine structure of lymphatic vascular-specific HS as a novel molecular target for strategies aiming to modulate DC behavior and\/or alter pathologic T cell responses in lymph nodes.<\/jats:p>","DOI":"10.4049\/jimmunol.1301286","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T01:30:23Z","timestamp":1391477423000},"page":"2133-2142","source":"Crossref","is-referenced-by-count":14,"title":["Lymphatic Specific Disruption in the Fine Structure of Heparan Sulfate Inhibits Dendritic Cell Traffic and Functional T Cell Responses in the Lymph Node"],"prefix":"10.1093","volume":"192","author":[{"given":"Xin","family":"Yin","sequence":"first","affiliation":[{"name":"Marine Drug Research Institute, Huaihai Institute of Technology , Lianyungang 222005,","place":["China"]},{"name":"Veterans Affairs San Diego Healthcare System, Medical and Research Services , San Diego, CA 92161"},{"name":"Division of Pulmonary and Critical Care, Department of Medicine, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott C","family":"Johns","sequence":"additional","affiliation":[{"name":"Veterans Affairs San Diego Healthcare System, Medical and Research Services , San Diego, CA 92161"},{"name":"Division of Pulmonary and Critical Care, Department of Medicine, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Kim","sequence":"additional","affiliation":[{"name":"Veterans Affairs San Diego Healthcare System, Medical and Research Services , San Diego, CA 92161"},{"name":"School of Medicine, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew","family":"Mikulski","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherina L","family":"Salanga","sequence":"additional","affiliation":[{"name":"Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmacology, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy M","family":"Handel","sequence":"additional","affiliation":[{"name":"Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmacology, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Macal","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elina\u00a0I","family":"Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark M","family":"Fuster","sequence":"additional","affiliation":[{"name":"Veterans Affairs San Diego Healthcare System, Medical and Research Services , San Diego, CA 92161"},{"name":"Division of Pulmonary and Critical Care, Department of Medicine, University of California San Diego , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,3,1]]},"reference":[{"key":"2025032404164089200_r1","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nri2297","article-title":"CCR7 and its ligands: balancing immunity and tolerance","volume":"8","author":"F\u00f6rster","year":"2008","journal-title":"Nat. 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