{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T22:30:31Z","timestamp":1775514631584,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The use of replication-deficient adenoviruses as vehicles for transfer of foreign genes offers many advantages in a vaccine setting, eliciting strong cellular immune responses involving both CD8+ and CD4+ T cells. Further improving the immunogenicity, tethering of the inserted target Ag to MHC class II\u2013associated invariant chain (Ii) greatly enhances both the presentation of most target Ags, as well as overall protection against viral infection, such as lymphocytic choriomeningitis virus (LCMV). The present study extends this vaccination concept to include protection against intracellular bacteria, using Listeria monocytogenes as a model organism. Protection in C57BL\/6 mice against recombinant L. monocytogenes expressing an immunodominant epitope of the LCMV glycoprotein (GP33) was greatly accelerated, augmented, and prolonged following vaccination with an adenoviral vaccine encoding GP linked to Ii compared with vaccination with the unlinked vaccine. Studies using knockout mice demonstrated that CD8+ T cells were largely responsible for this protection, which is mediated through perforin-dependent lysis of infected cells and IFN-\u03b3 production. Taking the concept a step further, vaccination of C57BL\/6 (L. monocytogenes\u2013resistant) and BALB\/c (L. monocytogenes\u2013susceptible) mice with adenoviral vectors encoding natural L. monocytogenes-derived soluble Ags (listeriolysin O and p60) revealed that tethering of these Ags to Ii markedly improved the vaccine-induced CD8+ T cell response to two of three epitopes studied. More importantly, Ii linkage accelerated and augmented vaccine-induced protection in both mouse strains and prolonged protection, in particular that induced by the weak Ag, p60, in L. monocytogenes\u2013susceptible BALB\/c mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1301290","type":"journal-article","created":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T04:14:53Z","timestamp":1379391293000},"page":"4152-4164","source":"Crossref","is-referenced-by-count":30,"title":["Adenovirus-Based Vaccine against <i>Listeria monocytogenes<\/i>: Extending the Concept of Invariant Chain Linkage"],"prefix":"10.1093","volume":"191","author":[{"given":"S\u00f8ren","family":"Jensen","sequence":"first","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Abildgaard","family":"Steffensen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin Anderschou Holbech","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Schl\u00fcter","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Medizinische Mikrobiologie, Otto-von-Guericke Universit\u00e4t Magdeburg , D-39106 Magdeburg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan Pravsgaard","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan Randrup","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,10,15]]},"reference":[{"key":"2025032405473270800_r1","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/35021239","article-title":"Vaccines against intracellular infections requiring cellular immunity","volume":"406","author":"Seder","year":"2000","journal-title":"Nature"},{"key":"2025032405473270800_r2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1086\/589862","article-title":"Vaccines: correlates of vaccine-induced immunity","volume":"47","author":"Plotkin","year":"2008","journal-title":"Clin. 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