{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:38:19Z","timestamp":1791275899365,"version":"4.1.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Plasmacytoid dendritic cells (pDCs) are considered to be the principal type-I IFN (IFN-I) source in response to viruses, whereas the contribution of conventional DCs (cDCs) has been underestimated because, on a per-cell basis, they are not considered professional IFN-I\u2013producing cells. We have investigated their respective roles in the IFN-I response required for CTL activation. Using a nonreplicative virus, baculovirus, we show that despite the high IFN-I\u2013producing abilities of pDCs, in vivo cDCs but not pDCs are the pivotal IFN-I producers upon viral injection, as demonstrated by selective pDC or cDC depletion. The pathway involved in the virus-triggered IFN-I response is dependent on TLR9\/MyD88 in pDCs and on stimulator of IFN genes (STING) in cDCs. Importantly, STING is the key molecule for the systemic baculovirus-induced IFN-I response required for CTL priming. The supremacy of cDCs over pDCs in fostering the IFN-I response required for CTL activation was also verified in the lymphocytic choriomeningitis virus model, in which IFN-\u03b2 promoter stimulator 1 plays the role of STING. However, when the TLR-independent virus-triggered IFN-I production is impaired, the pDC-induced IFNs-I have a primary impact on CTL activation, as shown by the detrimental effect of pDC depletion and IFN-I signaling blockade on the residual lymphocytic choriomeningitis virus\u2013triggered CTL response detected in IFN-\u03b2 promoter stimulator 1\u2212\/\u2212 mice. Our findings reveal that cDCs play a major role in the TLR-independent virus-triggered IFN-I production required for CTL priming, whereas pDC-induced IFNs-I are dispensable but become relevant when the TLR-independent IFN-I response is impaired.<\/jats:p>","DOI":"10.4049\/jimmunol.1301440","type":"journal-article","created":{"date-parts":[[2014,6,28]],"date-time":"2014-06-28T04:11:48Z","timestamp":1403928708000},"page":"1151-1161","source":"Crossref","is-referenced-by-count":25,"title":["Conventional but Not Plasmacytoid Dendritic Cells Foster the Systemic Virus\u2013Induced Type I IFN Response Needed for Efficient CD8 T Cell Priming"],"prefix":"10.1093","volume":"193","author":[{"given":"Sandra","family":"Hervas-Stubbs","sequence":"first","affiliation":[{"name":"Division of Immunology and Immunotherapy, Center for Applied Medical Research, University of Navarra , Pamplona 31008 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose-Ignacio","family":"Riezu-Boj","sequence":"additional","affiliation":[{"name":"Division of Immunology and Immunotherapy, Center for Applied Medical Research, University of Navarra , Pamplona 31008 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uxua","family":"Manche\u00f1o","sequence":"additional","affiliation":[{"name":"Division of Immunology and Immunotherapy, Center for Applied Medical Research, University of Navarra , Pamplona 31008 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Rueda","sequence":"additional","affiliation":[{"name":"Inmunolog\u00eda y Gen\u00e9tica Aplicada , S.A., Madrid 28037 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lissette","family":"Lopez","sequence":"additional","affiliation":[{"name":"Inmunolog\u00eda y Gen\u00e9tica Aplicada , S.A., Madrid 28037 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Alignani","sequence":"additional","affiliation":[{"name":"Division of Immunology and Immunotherapy, Center for Applied Medical Research, University of Navarra , Pamplona 31008 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Rodr\u00edguez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Division of Immunology and Immunotherapy, Center for Applied Medical Research, University of Navarra , Pamplona 31008 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Thieblemont","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 8147, Universit\u00e9 Ren\u00e9 Descartes Paris V, H\u00f4pital Necker , Paris F-75015 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Leclerc","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de R\u00e9gulation Immunitaire et Vaccinologie , Paris F-75015 ,","place":["France"]},{"name":"INSERM, Unit\u00e9 1041 , Paris F-75015 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,8,1]]},"reference":[{"key":"2025030707271758900_r1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.coi.2003.11.007","article-title":"Activation of dendritic cells: translating innate into adaptive immunity","volume":"16","author":"Reis e Sousa","year":"2004","journal-title":"Curr. 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