{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:20:36Z","timestamp":1791300036122,"version":"4.1.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We previously reported a peptide KS20 from islet amyloid polypeptide (IAPP) to be the target Ag for a highly diabetogenic CD4 T cell clone BDC-5.2.9. To track IAPP-reactive T cells in NOD mice and determine how they contribute to the pathogenesis of type 1 diabetes, we designed a new I-Ag7 tetramer with high affinity for BDC-5.2.9 that contains the peptide KS20. We found that significant numbers of KS20 tetramer+ CD4 T cells can be detected in the pancreas of prediabetic and diabetic NOD mice. To verify pathogenicity of IAPP-reactive cells, we sorted KS20 tetramer+ cells and cloned them from uncloned T cell lines isolated from spleen and lymph nodes of diabetic mice. We isolated a new KS20-reactive Th1 CD4 T cell clone that rapidly transfers diabetes. Our results suggest that IAPP triggers a broad autoimmune response by CD4 T cells in NOD mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1301480","type":"journal-article","created":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T04:14:53Z","timestamp":1379391293000},"page":"3990-3994","source":"Crossref","is-referenced-by-count":37,"title":["Cutting Edge: CD4 T Cells Reactive to an Islet Amyloid Polypeptide Peptide Accumulate in the Pancreas and Contribute to Disease Pathogenesis in Nonobese Diabetic Mice"],"prefix":"10.1093","volume":"191","author":[{"given":"Rocky L","family":"Baker","sequence":"first","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado at Denver School of Medicine and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Delong","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado at Denver School of Medicine and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene","family":"Barbour","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado at Denver School of Medicine and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Bradley","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado at Denver School of Medicine and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado at Denver School of Medicine and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Haskins","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado at Denver School of Medicine and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,10,15]]},"reference":[{"key":"2025032405480683600_r1","doi-asserted-by":"crossref","first-page":"7738","DOI":"10.4049\/jimmunol.175.11.7738","article-title":"Effector function of diabetogenic CD4 Th1 T cell clones: a central role for TNF-alpha","volume":"175","author":"Cantor","year":"2005","journal-title":"J. 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