{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T22:48:43Z","timestamp":1770677323149,"version":"3.49.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Thymocytes mature in a series of stages by migrating through specific areas of the thymus and interacting with other cells to receive the necessary developmental signals; however, little is known about the molecular mechanisms governing this migration. We report that murine thymocytes with a knockout mutation in \u03b1-PAK (p21-activated kinase)-interacting exchange factor (PIX; Arhgef6), an activator of Rho GTPases, showed greatly increased motility and altered morphology in two-dimensional migration on ICAM-1. \u03b1PIX was also required for efficient positive selection, but not negative selection, of thymocytes. TCR signaling was normal in \u03b1Pix\u2212 thymocytes, indicating that the effects of \u03b1PIX on positive selection are largely independent of TCR signaling. \u03b1Pix\u2212 thymocytes also paused less during migration in the thymic cortex, interacted less with ICAM-1 coated beads, and could overcome TCR stop signals, consistent with defective scanning behavior. These results identify \u03b1PIX as a regulator of thymocyte migration and subsequent arrest that is linked to positive selection.<\/jats:p>","DOI":"10.4049\/jimmunol.1302585","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T03:20:30Z","timestamp":1393903230000},"page":"3228-3238","source":"Crossref","is-referenced-by-count":10,"title":["\u03b1PIX RhoGEF Supports Positive Selection by Restraining Migration and Promoting Arrest of Thymocytes"],"prefix":"10.1093","volume":"192","author":[{"given":"Mark","family":"Korthals","sequence":"first","affiliation":[{"name":"Department of Functional Genomics and Medical Toponomics, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Schilling","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics and Medical Toponomics, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Reichardt","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Clinical Immunology, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dejan","family":"Mamula","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Schl\u00fcter","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Steiner","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Langn\u00e4se","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Thomas","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Neurobiology , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eckart","family":"Gundelfinger","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Neurobiology , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard T","family":"Premont","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center , Durham, NC 27701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerry","family":"Tedford","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics and Medical Toponomics, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus-Dieter","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics and Medical Toponomics, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]},{"name":"Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Medical Faculty , Magdeburg 39120,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,4,1]]},"reference":[{"key":"2025032404215074900_r1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/ni1323","article-title":"Thymic microenvironments for T cell differentiation and selection","volume":"7","author":"Ladi","year":"2006","journal-title":"Nat. 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