{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:33:16Z","timestamp":1791405196618,"version":"4.3.3"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chronic beryllium disease (CBD) is a granulomatous lung disease characterized by the accumulation of beryllium (Be)-specific CD4+ T cells in bronchoalveolar lavage. These expanded CD4+ T cells are composed of oligoclonal T cell subsets, suggesting their recruitment to the lung in response to conventional Ag. In the current study, we noted that all bronchoalveolar lavage\u2013derived T cell lines from HLA-DP2\u2013expressing CBD patients contained an expansion of Be-responsive V\u03b25.1+ CD4+ T cells. Using Be-loaded HLA-DP2\u2013peptide tetramers, the majority of tetramer-binding T cells also expressed V\u03b25.1 with a highly conserved CDR3\u03b2 motif. Interestingly, Be-specific, V\u03b25.1-expressing CD4+ T cells displayed differential HLA-DP2\u2013peptide tetramer staining intensity, and sequence analysis of the distinct tetramer-binding subsets showed that the two populations differed by a single conserved amino acid in the CDR3\u03b2 motif. TCR V\u03b1-chain analysis of purified V\u03b25.1+ CD4+ T cells based on differential tetramer-binding intensity showed differing TCR V\u03b1-chain pairing requirements, with the high-affinity population having promiscuous V\u03b1-chain pairing and the low-affinity subset requiring restricted V\u03b1-chain usage. Importantly, disease severity, as measured by loss of lung function, was inversely correlated with the frequency of tetramer-binding CD4+ T cells in the lung. Our findings suggest the presence of a dominant Be-specific, V\u03b25.1-expressing public T cell repertoire in the lungs of HLA-DP2\u2013expressing CBD patients using promiscuous V\u03b1-chain pairing to recognize an identical HLA-DP2-peptide\/Be complex. Importantly, the inverse relationship between expansion of CD4+ T cells expressing these public TCRs and disease severity suggests a pathogenic role for these T cells in CBD.<\/jats:p>","DOI":"10.4049\/jimmunol.1400007","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T03:28:12Z","timestamp":1397100492000},"page":"4571-4580","source":"Crossref","is-referenced-by-count":25,"title":["Identification of Multiple Public TCR Repertoires in Chronic Beryllium Disease"],"prefix":"10.1093","volume":"192","author":[{"given":"Natalie A","family":"Bowerman","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Colorado , Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T","family":"Falta","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado , Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas G","family":"Mack","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado , Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Wehrmann","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado , Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances","family":"Crawford","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, National Jewish Health , Denver, CO 80206"},{"name":"Howard Hughes Medical Institute and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret M","family":"Mroz","sequence":"additional","affiliation":[{"name":"Department of Medicine, National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A","family":"Maier","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado , Anschutz Medical Campus, Aurora, CO 80045"},{"name":"Department of Medicine, National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W","family":"Kappler","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, National Jewish Health , Denver, CO 80206"},{"name":"Howard Hughes Medical Institute and National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P","family":"Fontenot","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado , Anschutz Medical Campus, Aurora, CO 80045"},{"name":"Integrated Department of Immunology, National Jewish Health , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,5,15]]},"reference":[{"key":"2025032404291791000_r1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/cr.2012.1","article-title":"Determinants of public T cell responses","volume":"22","author":"Li","year":"2012","journal-title":"Cell Res."},{"key":"2025032404291791000_r2","doi-asserted-by":"crossref","first-page":"5782","DOI":"10.4049\/jimmunol.164.11.5782","article-title":"Size estimate of the \u03b1\u03b2 TCR repertoire of naive mouse splenocytes","volume":"164","author":"Casrouge","year":"2000","journal-title":"J. Immunol."},{"key":"2025032404291791000_r3","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nri2260","article-title":"The molecular basis for public T-cell responses?","volume":"8","author":"Venturi","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"2025032404291791000_r4","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1126\/science.286.5441.958","article-title":"A direct estimate of the human \u03b1\u03b2 T cell receptor diversity","volume":"286","author":"Arstila","year":"1999","journal-title":"Science"},{"key":"2025032404291791000_r5","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1084\/jem.20040638","article-title":"Limited T cell receptor diversity of HCV-specific T cell responses is associated with CTL escape","volume":"200","author":"Meyer-Olson","year":"2004","journal-title":"J. Exp. Med."},{"key":"2025032404291791000_r6","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/nri1977","article-title":"Structural determinants of T-cell receptor bias in immunity","volume":"6","author":"Turner","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"2025032404291791000_r7","doi-asserted-by":"crossref","first-page":"128ra142","DOI":"10.1126\/scitranslmed.3003647","article-title":"T cell receptor \u03b1\u03b2 diversity inversely correlates with pathogen-specific antibody levels in human cytomegalovirus infection","volume":"4","author":"Wang","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"2025032404291791000_r8","doi-asserted-by":"crossref","first-page":"e48117","DOI":"10.1371\/journal.pone.0048117","article-title":"Limited T cell receptor repertoire diversity in tuberculosis patients correlates with clinical severity","volume":"7","author":"Luo","year":"2012","journal-title":"PLoS ONE"},{"key":"2025032404291791000_r9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/ni1281","article-title":"Control of human immunodeficiency virus replication by cytotoxic T lymphocytes targeting subdominant epitopes","volume":"7","author":"Frahm","year":"2006","journal-title":"Nat. Immunol."},{"key":"2025032404291791000_r10","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.4049\/jimmunol.1001606","article-title":"Responses against a subdominant CD8+ T cell epitope protect against immunopathology caused by a dominant epitope","volume":"185","author":"Ruckwardt","year":"2010","journal-title":"J. Immunol."},{"key":"2025032404291791000_r11","doi-asserted-by":"crossref","first-page":"4829","DOI":"10.1074\/jbc.M110.191437","article-title":"T cell receptor clonotype influences epitope hierarchy in the CD8+ T cell response to respiratory syncytial virus infection","volume":"286","author":"Billam","year":"2011","journal-title":"J. Biol. Chem."},{"key":"2025032404291791000_r12","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.coi.2013.07.012","article-title":"T cell recognition of beryllium","volume":"25","author":"Dai","year":"2013","journal-title":"Curr. Opin. Immunol."},{"key":"2025032404291791000_r13","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.it.2005.08.004","article-title":"Genetic susceptibility and immune-mediated destruction in beryllium-induced disease","volume":"26","author":"Fontenot","year":"2005","journal-title":"Trends Immunol."},{"key":"2025032404291791000_r14","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1172\/JCI0215846","article-title":"Target organ localization of memory CD4+ T cells in patients with chronic beryllium disease","volume":"110","author":"Fontenot","year":"2002","journal-title":"J. Clin. Invest."},{"key":"2025032404291791000_r15","doi-asserted-by":"crossref","first-page":"518","DOI":"10.4049\/jimmunol.158.1.518","article-title":"Beryllium induces IL-2 and IFN-\u03b3 in berylliosis","volume":"158","author":"Tinkle","year":"1997","journal-title":"J. Immunol."},{"key":"2025032404291791000_r16","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1126\/science.8105536","article-title":"HLA-DPB1 glutamate 69: a genetic marker of beryllium disease","volume":"262","author":"Richeldi","year":"1993","journal-title":"Science"},{"key":"2025032404291791000_r17","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/(SICI)1097-0274(199710)32:4<337::AID-AJIM3>3.0.CO;2-R","article-title":"Interaction of genetic and exposure factors in the prevalence of berylliosis","volume":"32","author":"Richeldi","year":"1997","journal-title":"Am. J. Ind. Med."},{"key":"2025032404291791000_r18","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.4049\/jimmunol.163.3.1647","article-title":"Differential susceptibilities to chronic beryllium disease contributed by different Glu69 HLA-DPB1 and -DPA1 alleles","volume":"163","author":"Wang","year":"1999","journal-title":"J. Immunol."},{"key":"2025032404291791000_r19","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1164\/ajrccm.165.6.2104002","article-title":"Human leukocyte antigen class II amino acid epitopes: susceptibility and progression markers for beryllium hypersensitivity","volume":"165","author":"Rossman","year":"2002","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2025032404291791000_r20","doi-asserted-by":"crossref","first-page":"6910","DOI":"10.4049\/jimmunol.171.12.6910","article-title":"Influence of MHC class II in susceptibility to beryllium sensitization and chronic beryllium disease","volume":"171","author":"Maier","year":"2003","journal-title":"J. Immunol."},{"key":"2025032404291791000_r21","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/ajim.20045","article-title":"The association between HLA-DPB1Glu69 and chronic beryllium disease and beryllium sensitization","volume":"46","author":"McCanlies","year":"2004","journal-title":"Am. J. Ind. Med."},{"key":"2025032404291791000_r22","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/1465-9921-6-94","article-title":"Identification of HLA-DRPhebeta47 as the susceptibility marker of hypersensitivity to beryllium in individuals lacking the berylliosis-associated supratypic marker HLA-DPGlubeta69","volume":"6","author":"Amicosante","year":"2005","journal-title":"Respir. Res."},{"key":"2025032404291791000_r23","first-page":"175","article-title":"Role of the berylliosis-associated HLA-DPGlu69 supratypic variant in determining the response to beryllium in a blood T-cells beryllium-stimulated proliferation test","volume":"22","author":"Amicosante","year":"2005","journal-title":"Sarcoidosis Vasc. Diffuse Lung Dis."},{"key":"2025032404291791000_r24","doi-asserted-by":"crossref","first-page":"12717","DOI":"10.1073\/pnas.220430797","article-title":"Beryllium presentation to CD4+ T cells underlies disease-susceptibility HLA-DP alleles in chronic beryllium disease","volume":"97","author":"Fontenot","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032404291791000_r25","doi-asserted-by":"crossref","first-page":"3549","DOI":"10.4049\/jimmunol.166.5.3549","article-title":"HLA-DP allele-specific T cell responses to beryllium account for DP-associated susceptibility to chronic beryllium disease","volume":"166","author":"Lombardi","year":"2001","journal-title":"J. Immunol."},{"key":"2025032404291791000_r26","doi-asserted-by":"crossref","first-page":"3694","DOI":"10.4049\/jimmunol.1101872","article-title":"Mutagenesis of beryllium-specific TCRs suggests an unusual binding topology for antigen recognition","volume":"187","author":"Bowerman","year":"2011","journal-title":"J. Immunol."},{"key":"2025032404291791000_r27","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1084\/jem.20122426","article-title":"Identification of beryllium-dependent peptides recognized by CD4+ T cells in chronic beryllium disease","volume":"210","author":"Falta","year":"2013","journal-title":"J. Exp. Med."},{"key":"2025032404291791000_r28","doi-asserted-by":"crossref","first-page":"687","DOI":"10.7326\/0003-4819-108-5-687","article-title":"Proliferative response of bronchoalveolar lymphocytes to beryllium. A test for chronic beryllium disease","volume":"108","author":"Rossman","year":"1988","journal-title":"Ann. Intern. Med."},{"key":"2025032404291791000_r29","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1164\/ajrccm\/139.6.1479","article-title":"Pathologic and immunologic alterations in early stages of beryllium disease. Re-examination of disease definition and natural history","volume":"139","author":"Newman","year":"1989","journal-title":"Am. Rev. Respir. Dis."},{"key":"2025032404291791000_r30","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1164\/ajrccm\/148.3.661","article-title":"Early pulmonary physiologic abnormalities in beryllium disease","volume":"148","author":"Pappas","year":"1993","journal-title":"Am. Rev. Respir. Dis."},{"key":"2025032404291791000_r31","first-page":"455","article-title":"Human T-cell receptor variable gene segment families","volume":"42","author":"Arden","year":"1995","journal-title":"Immunogenetics"},{"key":"2025032404291791000_r32","doi-asserted-by":"crossref","first-page":"469","DOI":"10.2144\/000112123","article-title":"Amplification and analysis of cDNA generated from a single cell by 5\u2032-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells","volume":"40","author":"Ozawa","year":"2006","journal-title":"Biotechniques"},{"key":"2025032404291791000_r33","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.bbrc.2008.01.011","article-title":"Comprehensive analysis of the functional TCR repertoire at the single-cell level","volume":"367","author":"Ozawa","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2025032404291791000_r34","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1084\/jem.177.1.119","article-title":"Antigen recognition properties of mutant V\u03b2 3+ T cell receptors are consistent with an immunoglobulin-like structure for the receptor","volume":"177","author":"White","year":"1993","journal-title":"J. Exp. Med."},{"key":"2025032404291791000_r35","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1038\/ni1510","article-title":"Germline-encoded recognition of diverse glycolipids by natural killer T cells","volume":"8","author":"Scott-Browne","year":"2007","journal-title":"Nat. Immunol."},{"key":"2025032404291791000_r36","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1002\/art.21098","article-title":"Class II major histocompatibility complex-peptide tetramer staining in relation to functional avidity and T cell receptor diversity in the mouse CD4+ T cell response to a rheumatoid arthritis-associated antigen","volume":"52","author":"Falta","year":"2005","journal-title":"Arthritis Rheum."},{"key":"2025032404291791000_r37","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1165\/ajrcmb.18.4.2981","article-title":"Expansions of T-cell subsets expressing particular T-cell receptor variable regions in chronic beryllium disease","volume":"18","author":"Fontenot","year":"1998","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2025032404291791000_r38","doi-asserted-by":"crossref","first-page":"16729","DOI":"10.1073\/pnas.1113954108","article-title":"Specificity and detection of insulin-reactive CD4+ T cells in type 1 diabetes in the nonobese diabetic (NOD) mouse","volume":"108","author":"Crawford","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032404291791000_r39","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/ni1187","article-title":"Unconventional topology of self peptide-major histocompatibility complex binding by a human autoimmune T cell receptor","volume":"6","author":"Hahn","year":"2005","journal-title":"Nat. Immunol."},{"key":"2025032404291791000_r40","doi-asserted-by":"crossref","first-page":"15523","DOI":"10.1073\/pnas.0807338105","article-title":"Positioning of autoimmune TCR-Ob.2F3 and TCR-Ob.3D1 on the MBP85-99\/HLA-DR2 complex","volume":"105","author":"Kato","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032404291791000_r41","doi-asserted-by":"crossref","first-page":"2968","DOI":"10.1038\/sj.emboj.7600771","article-title":"Structure of a human autoimmune TCR bound to a myelin basic protein self-peptide and a multiple sclerosis-associated MHC class II molecule","volume":"24","author":"Li","year":"2005","journal-title":"EMBO J."},{"key":"2025032404291791000_r42","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1084\/jem.20100725","article-title":"A highly tilted binding mode by a self-reactive T cell receptor results in altered engagement of peptide and MHC","volume":"208","author":"Sethi","year":"2011","journal-title":"J. Exp. Med."},{"key":"2025032404291791000_r43","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1038\/emboj.2011.21","article-title":"Structure of a TCR with high affinity for self-antigen reveals basis for escape from negative selection","volume":"30","author":"Yin","year":"2011","journal-title":"EMBO J."},{"key":"2025032404291791000_r44","doi-asserted-by":"crossref","first-page":"4525","DOI":"10.4049\/jimmunol.166.7.4525","article-title":"The TCR repertoire of an immunodominant CD8+ T lymphocyte population","volume":"166","author":"Chen","year":"2001","journal-title":"J. Immunol."},{"key":"2025032404291791000_r45","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1006\/viro.2002.1743","article-title":"Clonotype tracking of TCR repertoires during chronic virus infections","volume":"304","author":"Cohen","year":"2002","journal-title":"Virology"},{"key":"2025032404291791000_r46","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1182\/blood-2005-04-1636","article-title":"Fluctuations of functionally distinct CD8+ T-cell clonotypes demonstrate flexibility of the HIV-specific TCR repertoire","volume":"107","author":"Meyer-Olson","year":"2006","journal-title":"Blood"},{"key":"2025032404291791000_r47","doi-asserted-by":"crossref","first-page":"6753","DOI":"10.4049\/jimmunol.1000812","article-title":"Broad cross-reactive TCR repertoires recognizing dissimilar Epstein-Barr and influenza A virus epitopes","volume":"185","author":"Clute","year":"2010","journal-title":"J. Immunol."},{"key":"2025032404291791000_r48","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.4049\/jimmunol.1102190","article-title":"Frequency of epitope-specific naive CD4+ T cells correlates with immunodominance in the human memory repertoire","volume":"188","author":"Kwok","year":"2012","journal-title":"J. Immunol."},{"key":"2025032404291791000_r49","doi-asserted-by":"crossref","first-page":"6334","DOI":"10.4049\/jimmunol.175.10.6334","article-title":"Ultrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining","volume":"175","author":"Scriba","year":"2005","journal-title":"J. Immunol."},{"key":"2025032404291791000_r50","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1365-2567.2008.03015.x","article-title":"T-cell receptor binding affinities and kinetics: impact on T-cell activity and specificity","volume":"126","author":"Stone","year":"2009","journal-title":"Immunology"},{"key":"2025032404291791000_r51","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1016\/j.molimm.2010.01.012","article-title":"T cell receptor CDR3 loops influence \u03b1\u03b2 pairing","volume":"47","author":"Bartok","year":"2010","journal-title":"Mol. Immunol."},{"key":"2025032404291791000_r52","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/j.molimm.2008.09.021","article-title":"Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain V\u03b1V\u03b2 fragments","volume":"46","author":"Richman","year":"2009","journal-title":"Mol. Immunol."},{"key":"2025032404291791000_r53","doi-asserted-by":"crossref","first-page":"7407","DOI":"10.4049\/jimmunol.181.10.7407","article-title":"Multiple glycines in TCR \u03b1-chains determine clonally diverse nature of human T cell memory to influenza A virus","volume":"181","author":"Naumov","year":"2008","journal-title":"J. Immunol."},{"key":"2025032404291791000_r54","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.immuni.2011.10.012","article-title":"A role for differential variable gene pairing in creating T cell receptors specific for unique major histocompatibility ligands","volume":"35","author":"Stadinski","year":"2011","journal-title":"Immunity"},{"key":"2025032404291791000_r55","doi-asserted-by":"crossref","first-page":"4285","DOI":"10.4049\/jimmunol.1003898","article-title":"A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing","volume":"186","author":"Venturi","year":"2011","journal-title":"J. Immunol."},{"key":"2025032404291791000_r56","doi-asserted-by":"crossref","first-page":"19414","DOI":"10.1073\/pnas.1010586107","article-title":"Convergent recombination shapes the clonotypic landscape of the naive T-cell repertoire","volume":"107","author":"Quigley","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032404291791000_r57","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.4049\/jimmunol.1002764","article-title":"Naive T cell repertoire skewing in HLA-A2 individuals by a specialized rearrangement mechanism results in public memory clonotypes","volume":"186","author":"Yassai","year":"2011","journal-title":"J. Immunol."},{"key":"2025032404291791000_r58","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1084\/jem.192.8.1191","article-title":"Genetic modulation of T cell receptor gene segment usage during somatic recombination","volume":"192","author":"Livak","year":"2000","journal-title":"J. Exp. Med."},{"key":"2025032404291791000_r59","doi-asserted-by":"crossref","first-page":"9536","DOI":"10.1073\/pnas.1106851108","article-title":"Structural basis for enabling T-cell receptor diversity within biased virus-specific CD8+ T-cell responses","volume":"108","author":"Day","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032404291791000_r60","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.jmb.2007.06.057","article-title":"CTL recognition of a protective immunodominant influenza A virus nucleoprotein epitope utilizes a highly restricted V\u03b2 but diverse V\u03b1 repertoire: functional and structural implications","volume":"372","author":"Zhong","year":"2007","journal-title":"J. Mol. Biol."},{"key":"2025032404291791000_r61","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0198-8859(96)00084-5","article-title":"Comparative sequence analysis of the human T cell receptor TCRA and TCRB CDR3 regions","volume":"48","author":"Moss","year":"1996","journal-title":"Hum. Immunol."},{"key":"2025032404291791000_r62","doi-asserted-by":"crossref","first-page":"4936","DOI":"10.4049\/jimmunol.1000173","article-title":"Evidence for a TCR affinity threshold delimiting maximal CD8 T cell function","volume":"184","author":"Schmid","year":"2010","journal-title":"J. Immunol."},{"key":"2025032404291791000_r63","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/ni863","article-title":"TCRs with high affinity for foreign pMHC show self-reactivity","volume":"4","author":"Holler","year":"2003","journal-title":"Nat. Immunol."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/192\/10\/4571\/62515221\/1400007.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/192\/10\/4571\/62515221\/1400007.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T11:17:28Z","timestamp":1742815048000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/192\/10\/4571\/8001482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5]]},"references-count":63,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,5,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1400007","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2014,5]]},"published":{"date-parts":[[2014,5]]}}}