{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:13:55Z","timestamp":1791209635747,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The Th1-associated chemokines CXCL9, CXCL10, and CXCL11 coordinate migration of CXCR3+ Th1 cells. The objective of this study was to evaluate the role of the innate immune system in stimulating chemokine expression in an experimental model of dry eye and bridge the gap between innate and adaptive immunity. Desiccating stress (DS) induced very early (6 h) expression and production of Th1-associated chemokines in cornea and conjunctiva of C57BL\/6 and RAG1 knockout (KO) mice, demonstrating that chemokine expression does not require innate T cells. We then demonstrated that activating the innate immune system prior to adoptive transfer of T cells to RAG1KO mice increased disease severity. Interestingly, lack of induction of chemokines CXCL9, CXCL10, and CXCL11 in IFN-\u03b3KO mice provided evidence that their expression requires IFN-\u03b3 for induction. Treatment of RAG1KO mice with anti-NK1.1 prevented the increase of CXCL9, CXCL10, and CXCL11 in response to DS, compared with isotype controls. Additionally, DS increased the expression of NKG2D in the conjunctiva. The expression of the NKG2D ligand, retinoic acid early inducible gene 1, also increased at the ocular surface at both the protein and gene levels. Neutralization of NKG2D at the ocular surface decreased the expression of CXCL9, CXCL10, CXCL11, and IFN-\u03b3. In summary, upregulation of CXCL9, CXCL10, and CXCL11 expression in experimental dry eye is T cell\u2013independent, requiring IFN-\u03b3\u2013producing NKG2D+ NK cells that are activated in response to DS-induced stress signals. This study provides insight into the events that trigger the initial immune response in dry eye pathology.<\/jats:p>","DOI":"10.4049\/jimmunol.1400016","type":"journal-article","created":{"date-parts":[[2014,10,7]],"date-time":"2014-10-07T07:44:17Z","timestamp":1412667857000},"page":"5264-5272","source":"Crossref","is-referenced-by-count":63,"title":["Desiccating Stress\u2013Induced Chemokine Expression in the Epithelium Is Dependent on Upregulation of NKG2D\/RAE-1 and Release of IFN-\u03b3 in Experimental Dry Eye"],"prefix":"10.1093","volume":"193","author":[{"given":"Terry G","family":"Coursey","sequence":"first","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Bohat","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia L","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen C","family":"Pflugfelder","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cintia S","family":"de Paiva","sequence":"additional","affiliation":[{"name":"Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,11,15]]},"reference":[{"key":"2025030707452348700_r1","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.4049\/jimmunol.176.7.3950","article-title":"Desiccating stress induces T cell-mediated Sj\u00f6gren\u2019s syndrome-like lacrimal keratoconjunctivitis","volume":"176","author":"Niederkorn","year":"2006","journal-title":"J. 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