{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T15:34:45Z","timestamp":1771342485413,"version":"3.50.1"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Adenoviral vectors have long been forerunners in the development of effective CD8 T cell\u2013based vaccines; therefore, it is imperative that we understand the factors controlling the induction of robust and long-lasting transgene-specific immune responses by these vectors. In this study, we investigated the organ sites, molecules, and cell subsets that play a critical role in the priming of transgene-specific CD8 T cells after vaccination with a replication-deficient adenoviral vector. Using a human adenovirus serotype 5 (Ad5) vector and genetically engineered mice, we found that CD8+ and\/or CD103+ dendritic cells in the draining lymph node played a critical role in the priming of Ad5-induced CD8 T cell responses. Moreover, we found that CD80\/86, but not CD28, was essential for efficient generation of both primary effectors and memory CD8 T cells. Interestingly, the lack of CD28 expression resulted in a delayed primary response, whereas memory CD8 T cells generated in CD28-deficient mice appeared almost normal in terms of both phenotype and effector cytokine profile, but they exhibited a significantly reduced proliferative capacity upon secondary challenge while retaining immediate in vivo effector capabilities: in vivo cytotoxicity and short-term in vivo protective capacity. Overall, our data point to an absolute requirement for professional APCs and the expression of the costimulatory molecules CD80\/86 for efficient CD8 T cell priming by adenoviral vectors. Additionally, our results suggest the existence of an alternative receptor for CD80\/86, which may substitute, in part, for CD28.<\/jats:p>","DOI":"10.4049\/jimmunol.1400197","type":"journal-article","created":{"date-parts":[[2014,6,21]],"date-time":"2014-06-21T08:05:10Z","timestamp":1403337910000},"page":"1223-1232","source":"Crossref","is-referenced-by-count":11,"title":["Priming of CD8 T Cells by Adenoviral Vectors Is Critically Dependent on B7 and Dendritic Cells but Only Partially Dependent on CD28 Ligation on CD8 T Cells"],"prefix":"10.1093","volume":"193","author":[{"given":"Karen N","family":"Nielsen","sequence":"first","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A","family":"Steffensen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan P","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan R","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology, and Microbiology, University of Copenhagen , DK-2200 Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,8,1]]},"reference":[{"key":"2025030707264405500_r1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1586\/erv.13.15","article-title":"Development of chimpanzee adenoviruses as vaccine vectors: challenges and successes emerging from clinical trials","volume":"12","author":"Capone","year":"2013","journal-title":"Expert Rev. 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