{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:51:03Z","timestamp":1790509863979,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC II) Ag-presenting molecules in lysosomes. Once MHC II molecules have bound a peptide, the MHC II\u2013peptide complex is delivered to the cell surface for presentation to CD4+ T cells. Regulation of Ag uptake via macropinocytosis and phagocytosis has been extensively studied, as well as trafficking in early endocytic vesicles notably regulated by the small GTPase Rab5 and its effectors. However, little is known about the regulators of Ag delivery from early endosomes to lysosomal compartments where the proper pH, proteases, MHC II, invariant chain, and HLA-DM reside, awaiting exogenous Ags for loading. In this article, we report the crucial role of the small GTPase ADP-ribosylation factor-like 8b (Arl8b) in MHC II presentation in DCs. We show for the first time, to our knowledge, that Arl8b localizes to MHC II compartments in DCs and regulates formation of MHC II\u2013peptide complexes. Arl8b-silenced DCs display a defect in MHC II\u2013Ag complex formation and its delivery to the cell surface during infection resulting in a defect in T cell recognition. Our results highlight the role of Arl8b as a trafficking regulator of the late stage of complex formation and MHC II presentation in DCs.<\/jats:p>","DOI":"10.4049\/jimmunol.1401072","type":"journal-article","created":{"date-parts":[[2015,1,31]],"date-time":"2015-01-31T00:17:48Z","timestamp":1422663468000},"page":"2079-2088","source":"Crossref","is-referenced-by-count":43,"title":["MHC Class II Presentation Is Controlled by the Lysosomal Small GTPase, Arl8b"],"prefix":"10.1093","volume":"194","author":[{"given":"Xavier","family":"Michelet","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women\u2019s Hospital, Harvard Medical School , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salil","family":"Garg","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women\u2019s Hospital, Harvard Medical School , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin J","family":"Wolf","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women\u2019s Hospital, Harvard Medical School , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Tuli","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women\u2019s Hospital, Harvard Medical School , Boston, MA 02115"},{"name":"Division of Cell Biology and Immunology, Institute of Microbial Technology , Chandigarh 160036 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Ricciardi-Castagnoli","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network, Agency for Science, Technology and Research , 138648 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B","family":"Brenner","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women\u2019s Hospital, Harvard Medical School , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,3,1]]},"reference":[{"key":"2025030705114915100_r1","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1146\/annurev.immunol.22.012703.104538","article-title":"Cell biology of antigen processing in vitro and in vivo","volume":"23","author":"Trombetta","year":"2005","journal-title":"Annu. 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