{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:38:59Z","timestamp":1791268739776,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mouse experimental autoimmune glomerulonephritis, a model of human antiglomerular basement membrane disease, depends on both Ab and T cell responses to the Goodpasture Ag noncollagenous domain 1 of the \u03b13-chain of type IV collagen (\u03b13IV-NC1). The aim of our study was to further characterize the T cell\u2013mediated immune response. Repeated immunization with mouse \u03b13IV-NC1 caused fatal glomerulonephritis in DBA\/1 mice. Although two immunizations were sufficient to generate high \u03b13IV-NC1\u2013specific IgG titers, Ab and complement deposition along the glomerular basement membranes, and a nephrotic syndrome, two additional immunizations were needed to induce a necrotizing\/crescentic glomerulonephritis. Ten days after the first immunization, \u03b13IV-NC1\u2013specific CD4+ cells producing TNF-\u03b1, IFN-\u03b3, or IL-17A were detected in the spleen. With the emergence of necrotizing\/crescentic glomerulonephritis, \u223c0.15% of renal CD4+ cells were specific for \u03b13IV-NC1. Using peptides spanning the whole \u03b13IV-NC1 domain, three immunodominant T cell epitopes were identified. Immunization with these peptides did not lead to clinical signs of experimental autoimmune glomerulonephritis or necrotizing\/crescentic glomerulonephritis. However, mice immunized with one of the peptides (STVKAGDLEKIISRC) developed circulating Abs against mouse \u03b13IV-NC1 first detected at 8 wk, and 50% of the mice showed mild proteinuria at 18\u201324 wk due to membranous glomerulopathy. Taken together, our results suggest that autoreactive T cells are able to induce the formation of pathologic autoantibodies. The quality and quantity of \u03b13IV-NC1\u2013specific Ab and T cell responses are critical for the phenotype of the glomerulonephritis.<\/jats:p>","DOI":"10.4049\/jimmunol.1401267","type":"journal-article","created":{"date-parts":[[2015,3,14]],"date-time":"2015-03-14T03:01:48Z","timestamp":1426302108000},"page":"3646-3655","source":"Crossref","is-referenced-by-count":14,"title":["Glomerulopathy Induced by Immunization with a Peptide Derived from the Goodpasture Antigen \u03b13IV-NC1"],"prefix":"10.1093","volume":"194","author":[{"given":"Helmut","family":"Hopfer","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Hospital Basel , 4031 Basel ,","place":["Switzerland"]},{"name":"Institute of Pathology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"H\u00fcnem\u00f6rder","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Treder","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-Eric","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Joachim","family":"Paust","sequence":"additional","affiliation":[{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Meyer-Schwesinger","sequence":"additional","affiliation":[{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Hopfer","sequence":"additional","affiliation":[{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlies","family":"Sachs","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]},{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anett","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biranda","family":"Bucher-Kocaoglu","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Ahrens","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Panzer","sequence":"additional","affiliation":[{"name":"Department of Medicine, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Willi","family":"Mittr\u00fccker","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University Medical Center Hamburg\u2013Eppendorf , 20246 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,4,15]]},"reference":[{"key":"2025030706021897500_r1","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1056\/NEJMra022296","article-title":"Alport\u2019s syndrome, Goodpasture\u2019s syndrome, and type IV collagen","volume":"348","author":"Hudson","year":"2003","journal-title":"N. 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