{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:07:54Z","timestamp":1791198474182,"version":"4.1.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Human circulating Ag-induced plasma cells (PCs) contain a high proportion of cycling cells. This study reveals that these PCs spontaneously proliferate in culture during 72 h, as determined by BrdU-uptake detection. Transcriptome analysis indicates that, in comparison with tonsil and bone marrow (BM) PCs, these PCs distinctively upregulate genes involved in cell division. Blood PC proliferation occurs simultaneously with increasing apoptosis rates, and is associated with PC survival. In addition, the proliferating activity of these PCs is enhanced by the addition of cytokines present in PC survival niches. Moreover, blood Ag-induced, but not BM, PCs exhibit the expression of molecules involved in the interaction between memory B cells and T follicular helper (Tfh) cells. In fact, purified circulating and tonsil Tfh cells increased IgG secretion by blood Ag-induced, but not by BM, PCs. This effect is exerted by augmenting blood PC survival through a mechanism partly dependent on cell contact. These results strongly suggest that the proliferating capacity of circulating Ag-induced PCs contributes to their competitive migration to survival niches, either to long-living PC niches or to temporal niches present in reactive lymphoid organs and inflamed tissues, structures where Tfh cells appear to participate.<\/jats:p>","DOI":"10.4049\/jimmunol.1402231","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T08:27:26Z","timestamp":1419928046000},"page":"1031-1038","source":"Crossref","is-referenced-by-count":11,"title":["Survival of Human Circulating Antigen-Induced Plasma Cells Is Supported by Plasma Cell\u2013Niche Cytokines and T Follicular Helper Lymphocytes"],"prefix":"10.1093","volume":"194","author":[{"given":"Ana","family":"Ramos-Amaya","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n, Hospital Universitario Puerta del Mar , 11009 Cadiz ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Rodr\u00edguez-Bayona","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n, Hospital Universitario Puerta del Mar , 11009 Cadiz ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n","family":"L\u00f3pez-Blanco","sequence":"additional","affiliation":[{"name":"Servicio de Inmunolog\u00eda, Hospital Universitario Puerta del Mar , 11009 Cadiz ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloisa","family":"And\u00fajar","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00f3mica, Centro Andaluz de Biolog\u00eda Molecular y Medicina Regenerativa , 41092 Seville ,","place":["Spain"]},{"name":"Consejo Superior de Investigaciones Cient\u00edficas , 28008 Madrid ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"P\u00e9rez-Alegre","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00f3mica, Centro Andaluz de Biolog\u00eda Molecular y Medicina Regenerativa , 41092 Seville ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Campos-Caro","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n, Hospital Universitario Puerta del Mar , 11009 Cadiz ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A","family":"Brieva","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n, Hospital Universitario Puerta del Mar , 11009 Cadiz ,","place":["Spain"]},{"name":"Servicio de Inmunolog\u00eda, Hospital Universitario Puerta del Mar , 11009 Cadiz ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,2,1]]},"reference":[{"key":"2025030704591529900_r1","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1002\/eji.1830211209","article-title":"Sites of specific B cell activation in primary and secondary responses to T cell-dependent and T cell-independent antigens","volume":"21","author":"Liu","year":"1991","journal-title":"Eur. 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