{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T17:11:56Z","timestamp":1787332316221,"version":"build-2736575974"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>MicroRNA (miR)-mediated regulation of protein abundance is a pervasive mechanism of directing cellular processes. The well-studied and abundant miR-182 has previously been implicated in many aspects of T cell function, DNA repair, and cancer. In this study, we show that miR-182 is the most highly induced miR in B cells undergoing class-switch recombination. To elucidate the requirement of miR-182 in lymphocyte function, we extensively characterized mice with a targeted deletion of Mir182. We show that despite its dramatic induction, loss of miR-182 has minimal impact on B cell development, the ability of B cells to undergo class-switch recombination ex vivo and to undergo Ag-driven affinity maturation in vivo. Furthermore, in striking contrast to knockdown studies that demonstrated the requirement of miR-182 in T cell function, miR-182\u2013deficient mice display no defect in T cell development and activation. Finally, we show that T cell\u2013dependent immune response to experimental Listeria monocytogenes infection is intact in miR-182\u2013deficient mice. We conclude that, contrary to previous studies, miR-182 does not play a significant role in all measured aspects of mouse adaptive immunity. This striking absence of a phenotype highlights the lack of correlation between expression pattern and functional requirement, underscores the limitations of using knockdown approaches to assess miR requirements, and suggests that miR networks may compensate for the chronic loss of specific miRs.<\/jats:p>","DOI":"10.4049\/jimmunol.1402261","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T06:11:17Z","timestamp":1423721477000},"page":"2635-2642","source":"Crossref","is-referenced-by-count":29,"title":["miR-182 Is Largely Dispensable for Adaptive Immunity: Lack of Correlation between Expression and Function"],"prefix":"10.1093","volume":"194","author":[{"given":"Joseph N","family":"Pucella","sequence":"first","affiliation":[{"name":"Immunology Program, Memorial Sloan Kettering Cancer Center, Gerstner Sloan Kettering Graduate School , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Feng","family":"Yen","sequence":"additional","affiliation":[{"name":"Immunology Program, Memorial Sloan Kettering Cancer Center, Gerstner Sloan Kettering Graduate School , New York, NY 10065"},{"name":"Immunology and Microbial Pathogenesis Program, Weill\u2013Cornell Graduate School of Medical Sciences , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myoungjoo V","family":"Kim","sequence":"additional","affiliation":[{"name":"Immunology Program, Memorial Sloan Kettering Cancer Center, Gerstner Sloan Kettering Graduate School , New York, NY 10065"},{"name":"Immunology and Microbial Pathogenesis Program, Weill\u2013Cornell Graduate School of Medical Sciences , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris","family":"van der Veeken","sequence":"additional","affiliation":[{"name":"Immunology Program, Memorial Sloan Kettering Cancer Center, Gerstner Sloan Kettering Graduate School , New York, NY 10065"},{"name":"Immunology and Microbial Pathogenesis Program, Weill\u2013Cornell Graduate School of Medical Sciences , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas D","family":"Socci","sequence":"additional","affiliation":[{"name":"Bioinformatics Core, Memorial Sloan Kettering Cancer Center , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, National Cerebral and Cardiovascular Center , Suita, Osaka 565-8565 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming O","family":"Li","sequence":"additional","affiliation":[{"name":"Immunology Program, Memorial Sloan Kettering Cancer Center, Gerstner Sloan Kettering Graduate School , New York, NY 10065"},{"name":"Immunology and Microbial Pathogenesis Program, Weill\u2013Cornell Graduate School of Medical Sciences , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoharu","family":"Iwai","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, National Cerebral and Cardiovascular Center , Suita, Osaka 565-8565 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayanta","family":"Chaudhuri","sequence":"additional","affiliation":[{"name":"Immunology Program, Memorial Sloan Kettering Cancer Center, Gerstner Sloan Kettering Graduate School , New York, NY 10065"},{"name":"Immunology and Microbial Pathogenesis Program, Weill\u2013Cornell Graduate School of Medical Sciences , New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,3,15]]},"reference":[{"key":"2025030705253879700_r1","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.it.2011.03.003","article-title":"Complex regulation and function of activation-induced cytidine deaminase","volume":"32","author":"Stavnezer","year":"2011","journal-title":"Trends Immunol."},{"key":"2025030705253879700_r2","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/nature06547","article-title":"Two levels of protection for the B cell genome during somatic hypermutation","volume":"451","author":"Liu","year":"2008","journal-title":"Nature"},{"key":"2025030705253879700_r3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-800267-4.00001-8","article-title":"Regulation of immunoglobulin class-switch recombination: choreography of noncoding transcription, targeted DNA deamination, and long-range DNA repair","volume":"122","author":"Matthews","year":"2014","journal-title":"Adv. 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