{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T17:51:32Z","timestamp":1769277092720,"version":"3.49.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Burkholderia pseudomallei is the causative agent of melioidosis characterized by pneumonia and fatal septicemia and prevalent in Southeast Asia. Related Burkholderia species are strong risk factors of mortality in cystic fibrosis (CF). The B. \u00a0pseudomallei flagellar protein FliC is strongly seroreactive and vaccination protects challenged mice. We assessed B. \u00a0pseudomallei FliC peptide binding affinity to multiple HLA class II alleles and then assessed CD4 T cell immunity in HLA class II transgenic mice and in seropositive individuals in Thailand. T cell hybridomas were generated to investigate cross-reactivity between B. \u00a0pseudomallei and the related Burkholderia species associated with Cepacia Complex CF. B. \u00a0pseudomallei FliC contained several peptide sequences with ability to bind multiple HLA class II alleles. Several peptides were shown to encompass strong CD4 T cell epitopes in B. \u00a0pseudomallei\u2013exposed individuals and in HLA transgenic mice. In particular, the p38 epitope is robustly recognized by CD4 T cells of seropositive donors across diverse HLA haplotypes. T cell hybridomas against an immunogenic B. \u00a0pseudomallei FliC epitope also cross-reacted with orthologous FliC sequences from Burkholderia multivorans and Burkholderia cenocepacia, important pathogens in CF. Epitopes within FliC were accessible for processing and presentation from live or heat-killed bacteria, demonstrating that flagellin enters the HLA class II Ag presentation pathway during infection of macrophages with B. cenocepacia. Collectively, the data support the possibility of incorporating FliC T cell epitopes into vaccination programs targeting both at-risk individuals in B. pseudomallei endemic regions as well as CF patients.<\/jats:p>","DOI":"10.4049\/jimmunol.1402273","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T04:24:01Z","timestamp":1415852641000},"page":"6041-6049","source":"Crossref","is-referenced-by-count":23,"title":["CD4+ T Cell Epitopes of FliC Conserved between Strains of <i>Burkholderia<\/i>: Implications for Vaccines against Melioidosis and Cepacia Complex in Cystic Fibrosis"],"prefix":"10.1093","volume":"193","author":[{"given":"Julie A","family":"Musson","sequence":"first","affiliation":[{"name":"Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne , Tyne and Wear NE2 4HH ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine J","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Immunity, Department of Medicine, Imperial College , London W12 0NN ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darawan","family":"Rinchai","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University , Khon Kaen 40002 ,","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnone","family":"Nithichanon","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University , Khon Kaen 40002 ,","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasong","family":"Khaenam","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University , Khon Kaen 40002 ,","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Favry","sequence":"additional","affiliation":[{"name":"Commiseriat \u00e0 l'Energie Atomique, Insititut de Biologie et de Technologies de Saclay, Service d'Ing\u00e9nierie Mol\u00e9culaire des Prot\u00e9ines (SIMOPRO) , 91191 Gif Sur Yvette ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Spink","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine , London WC1E 7HT ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen K Y","family":"Chu","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Immunity, Department of Medicine, Imperial College , London W12 0NN ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"De Soyza","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne , Tyne and Wear NE2 4HH ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J","family":"Bancroft","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine , London WC1E 7HT ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganjana","family":"Lertmemongkolchai","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University , Khon Kaen 40002 ,","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Maillere","sequence":"additional","affiliation":[{"name":"Commiseriat \u00e0 l'Energie Atomique, Insititut de Biologie et de Technologies de Saclay, Service d'Ing\u00e9nierie Mol\u00e9culaire des Prot\u00e9ines (SIMOPRO) , 91191 Gif Sur Yvette ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemary J","family":"Boyton","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Immunity, Department of Medicine, Imperial College , London W12 0NN ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel M","family":"Altmann","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Immunity, Department of Medicine, Imperial College , London W12 0NN ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H","family":"Robinson","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne , Tyne and Wear NE2 4HH ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,12,15]]},"reference":[{"key":"2025030707511293300_r1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1146\/annurev.micro.112408.134030","article-title":"Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis","volume":"64","author":"Galyov","year":"2010","journal-title":"Annu. 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