{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T19:33:55Z","timestamp":1771875235572,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although reports documented aberrant cytokine expression in autoimmune bullous dermatoses (AIBDs), cytokine-targeting therapies have not been established in these disorders. We showed previously that IL-6 treatment protected against tissue destruction in experimental epidermolysis bullosa acquisita (EBA), an AIBD caused by autoantibodies to type VII collagen (COL7). The anti-inflammatory effects of IL-6 were mediated by induction of IL-1ra, and prophylactic IL-1ra administration prevented blistering. In this article, we demonstrate elevated serum concentrations of IL-1\u03b2 in both mice with experimental EBA induced by injection of anti-COL7 IgG and in EBA patients. Increased IL-1\u03b1 and IL-1\u03b2 expression also was observed in the skin of anti-COL7 IgG-injected wild-type mice compared with the significantly less diseased IL-1R\u2013deficient or wild-type mice treated with the IL-1R antagonist anakinra or anti\u2013IL-1\u03b2. These findings suggested that IL-1 contributed to recruitment of inflammatory cells into the skin. Accordingly, the expression of ICAM-1 was decreased in IL-1R\u2013deficient and anakinra-treated mice injected with anti-COL7. This effect appeared to be specifically attributable to IL-1 because anakinra blocked the upregulation of different endothelial adhesion molecules on IL-1\u2013stimulated, but not on TNF-\u03b1\u2013stimulated, cultured endothelial cells. Interestingly, injection of caspase-1\/11\u2013deficient mice with anti-COL7 IgG led to the same extent of skin lesions as in wild-type mice. Collectively, our data suggest that IL-1, independently of caspase-1, contributes to the pathogenesis of EBA. Because anti\u2013IL-1\u03b2 in a prophylactic setting and anakinra in a quasi-therapeutic setting (i.e., when skin lesions had already developed) improved experimental EBA, IL-1 appears to be a potential therapeutic target for EBA and related AIBDs.<\/jats:p>","DOI":"10.4049\/jimmunol.1402688","type":"journal-article","created":{"date-parts":[[2015,3,21]],"date-time":"2015-03-21T03:56:59Z","timestamp":1426910219000},"page":"3656-3663","source":"Crossref","is-referenced-by-count":42,"title":["Caspase-1\u2013Independent IL-1 Release Mediates Blister Formation in Autoantibody-Induced Tissue Injury through Modulation of Endothelial Adhesion Molecules"],"prefix":"10.1093","volume":"194","author":[{"given":"Hengameh","family":"Sadeghi","sequence":"first","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anike","family":"Lockmann","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Allergology, University Medical Center , 37075 G\u00f6ttingen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna-Carina","family":"Hund","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Allergology, University Medical Center , 37075 G\u00f6ttingen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Unni K S R L","family":"Samavedam","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]},{"name":"L\u00fcbeck Institute of Experimental Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Pipi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]},{"name":"L\u00fcbeck Institute of Experimental Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Vafia","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Hauenschild","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of L\u00fcbeck , 23562 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Kalies","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of L\u00fcbeck , 23562 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hendri H","family":"Pas","sequence":"additional","affiliation":[{"name":"Center for Blistering Diseases, Department of Dermatology, University Medical Center Groningen, University of Groningen , 9700 RB Groningen ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel F","family":"Jonkman","sequence":"additional","affiliation":[{"name":"Center for Blistering Diseases, Department of Dermatology, University Medical Center Groningen, University of Groningen , 9700 RB Groningen ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Recke","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]},{"name":"L\u00fcbeck Institute of Experimental Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P","family":"Sch\u00f6n","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Allergology, University Medical Center , 37075 G\u00f6ttingen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Detlef","family":"Zillikens","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]},{"name":"L\u00fcbeck Institute of Experimental Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enno","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]},{"name":"L\u00fcbeck Institute of Experimental Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf J","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck ,","place":["Germany"]},{"name":"L\u00fcbeck Institute of Experimental Dermatology, University of L\u00fcbeck , 23538 L\u00fcbeck 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