{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T20:16:19Z","timestamp":1775506579860,"version":"3.50.1"},"reference-count":96,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The IL-2 receptor \u03b3 common (IL-2R\u03b3c) chain is the shared subunit of the receptors for the IL-2 family of cytokines, which mediate signaling through JAK3 and various downstream pathways to regulate lymphopoiesis. Inactivating mutations in human IL-2R\u03b3c result in SCID, a primary immunodeficiency characterized by greatly reduced numbers of lymphocytes. This study used bioinformatics, expression analysis, gene ablation, and specific pharmacologic inhibitors to investigate the function of two putative zebrafish IL-2R\u03b3c paralogs, il-2r\u03b3c.a and il-2r\u03b3c.b, and downstream signaling components during early lymphopoiesis. Expression of il-2r\u03b3c.a commenced at 16 h post fertilization (hpf) and rose steadily from 4\u20136 d postfertilization (dpf) in the developing thymus, with il-2r\u03b3c.a expression also confirmed in adult T and B lymphocytes. Transcripts of il-2r\u03b3c.b were first observed from 8 hpf, but waned from 16 hpf before reaching maximal expression at 6 dpf, but this was not evident in the thymus. Knockdown of il-2r\u03b3c.a, but not il-2r\u03b3c.b, substantially reduced embryonic lymphopoiesis without affecting other aspects of hematopoiesis. Specific targeting of zebrafish Jak3 exerted a similar effect on lymphopoiesis, whereas ablation of zebrafish Stat5.1 and pharmacologic inhibition of PI3K and MEK also produced significant but smaller effects. Ablation of il-2r\u03b3c.a was further demonstrated to lead to an absence of mature T cells, but not B cells in juvenile fish. These results indicate that conserved IL-2R\u03b3c signaling via JAK3 plays a key role during early zebrafish lymphopoiesis, which can be potentially targeted to generate a zebrafish model of human SCID.<\/jats:p>","DOI":"10.4049\/jimmunol.1403060","type":"journal-article","created":{"date-parts":[[2015,11,21]],"date-time":"2015-11-21T04:03:50Z","timestamp":1448078630000},"page":"135-143","source":"Crossref","is-referenced-by-count":23,"title":["Conserved IL-2R\u03b3c Signaling Mediates Lymphopoiesis in Zebrafish"],"prefix":"10.1093","volume":"196","author":[{"given":"Robert","family":"Sertori","sequence":"first","affiliation":[{"name":"School of Medicine, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]},{"name":"Centre for Molecular and Medical Research, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1646-4905","authenticated-orcid":false,"given":"Clifford","family":"Liongue","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]},{"name":"Centre for Molecular and Medical Research, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faiza","family":"Basheer","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]},{"name":"Centre for Molecular and Medical Research, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanako L","family":"Lewis","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California , San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parisa","family":"Rasighaemi","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]},{"name":"Centre for Molecular and Medical Research, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0575-1554","authenticated-orcid":false,"given":"Dennis","family":"de Coninck","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]},{"name":"GROW School for Oncology and Developmental Biology, Maastricht University , Maastricht 6200 ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Traver","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California , San Diego, La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alister C","family":"Ward","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]},{"name":"Centre for Molecular and Medical Research, Deakin University , Geelong, Victoria 3216 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,1,1]]},"reference":[{"key":"2025030702055322700_r1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.imlet.2006.11.006","article-title":"Common \u03b3 chain cytokines: dissidence in the details","volume":"108","author":"Alves","year":"2007","journal-title":"Immunol. 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