{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:17:31Z","timestamp":1782274651821,"version":"3.54.5"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>TLR7 recognizes pathogen-derived and self-derived RNA, and thus a regulatory system for control of the TLR7 response is required to avoid excessive activation. Unc93 homolog B1 (Unc93B1) is a regulator of TLR7 that controls the TLR7 response by transporting TLR7 from the endoplasmic reticulum to endolysosomes. We have previously shown that a D34A mutation in Unc93B1 induces hyperactivation of TLR7, and that Unc93b1D34A\/D34A mice (D34A mice) have systemic inflammation spontaneously. In this study, we examined the roles of inflammatory cytokines such as IFN-\u03b3, IL-17A, and type I IFNs to understand the mechanism underlying the phenotype in D34A mice. mRNAs for IFN-\u03b3 and IL-I7A in CD4+ T cells increased, but inflammatory phenotype manifesting as thrombocytopenia and splenomegaly was still observed in Ifng\u2212\/\u2212 or Il17a\u2212\/\u2212 D34A mice. In contrast to T cell\u2013derived cytokines, Ifnar1\u2212\/\u2212 D34A mice showed an ameliorated phenotype with lower expression of TLR7 in B cells and conventional dendritic cells (cDCs). The amount of TLR7 decreased in B cells from Ifnar1\u2212\/\u2212 D34A mice, but the percentage of TLR7+ cells decreased among CD8\u03b1\u2212 cDCs. In conclusion, type I IFNs maintain expression of TLR7 in B cells and cDCs in different ways; total amount of TLR7 is kept in B cells and TLR7+ population is retained among cDCs. Our results suggested that these TLR7-expressing cells are activated initially and influence TLR7-dependent systemic inflammation.<\/jats:p>","DOI":"10.4049\/jimmunol.1500071","type":"journal-article","created":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T03:38:37Z","timestamp":1448941117000},"page":"416-427","source":"Crossref","is-referenced-by-count":7,"title":["Type I IFN Contributes to the Phenotype of <i>Unc93b1<\/i>D34A\/D34A Mice by Regulating TLR7 Expression in B Cells and Dendritic Cells"],"prefix":"10.1093","volume":"196","author":[{"given":"Ryutaro","family":"Fukui","sequence":"first","affiliation":[{"name":"Division of Innate Immunity, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo , Tokyo 108-8639 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuo","family":"Kanno","sequence":"additional","affiliation":[{"name":"Division of Innate Immunity, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo , Tokyo 108-8639 ,","place":["Japan"]},{"name":"Laboratory of Innate Immunity, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo , Tokyo 108-8639 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Miyake","sequence":"additional","affiliation":[{"name":"Division of Innate Immunity, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo , Tokyo 108-8639 ,","place":["Japan"]},{"name":"Laboratory of Innate Immunity, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo , Tokyo 108-8639 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,1,1]]},"reference":[{"key":"2025030702105238200_r1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/ni.1863","article-title":"The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors","volume":"11","author":"Kawai","year":"2010","journal-title":"Nat. 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