{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:17:11Z","timestamp":1790371031913,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>B cells play a critical role in the clearance of Pneumocystis. In addition to production of Pneumocystis-specific Abs, B cells are required during the priming phase for CD4+ T cells to expand normally and generate memory. Clearance of Pneumocystis was found to be dependent on Ag specific B cells and on the ability of B cells to secrete Pneumocystis-specific Ab, as mice with B cells defective in these functions or with a restricted BCR were unable to control Pneumocystis infection. Because Pneumocystis-specific antiserum was only able to partially protect B cell\u2013deficient mice from infection, we hypothesized that optimal T cell priming requires fully functional B cells. Using adoptive transfer and B cell depletion strategies, we determined that optimal priming of CD4+ T cells requires B cells during the first 2\u20133 d of infection and that this was independent of the production of Ab. T cells that were removed from Pneumocystis-infected mice during the priming phase were fully functional and able to clear Pneumocystis infection upon adoptive transfer into Rag1\u2212\/\u2212 hosts, but this effect was ablated in mice that lacked fully functional B cells. Our results indicate that T cell priming requires a complete environment of Ag presentation and activation signals to become fully functional in this model of Pneumocystis infection.<\/jats:p>","DOI":"10.4049\/jimmunol.1500112","type":"journal-article","created":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T11:15:25Z","timestamp":1433416525000},"page":"611-620","source":"Crossref","is-referenced-by-count":45,"title":["B Lymphocytes Are Required during the Early Priming of CD4+ T Cells for Clearance of <i>Pneumocystis<\/i> Infection in Mice"],"prefix":"10.1093","volume":"195","author":[{"given":"Michael M","family":"Opata","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa L","family":"Hollifield","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances E","family":"Lund","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy D","family":"Randall","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Dunn","sequence":"additional","affiliation":[{"name":"Genomics Institute of the Novartis Research Foundation , San Diego, CA 92121"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth A","family":"Garvy","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J","family":"Feola","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice and Science, University of Kentucky College of Pharmacy , Lexington, KY 40536"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,7,15]]},"reference":[{"key":"2025030703435700600_r1","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.3201\/eid1010.030985","article-title":"Current epidemiology of Pneumocystis pneumonia","volume":"10","author":"Morris","year":"2004","journal-title":"Emerg. 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