{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:47:26Z","timestamp":1777650446620,"version":"3.51.4"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Following T cell encounter with Ag, multiple signals are integrated to collectively induce distinct differentiation programs within Ag-specific CD8+ T cell populations. Several factors contribute to these cell fate decisions, including the amount and duration of Ag, exposure to inflammatory cytokines, and degree of ligation of cosignaling molecules. The ICOS is not expressed on resting T cells but is rapidly upregulated upon encounter with Ag. However, the impact of ICOS signaling on programmed differentiation is not well understood. In this study, we therefore sought to determine the role of ICOS signaling on CD8+ T cell programmed differentiation. Through the creation of novel ICOS retrogenic Ag-specific TCR-transgenic CD8+ T cells, we interrogated the phenotype, functionality, and recall potential of CD8+ T cells that receive early and sustained ICOS signaling during Ag exposure. Our results reveal that these ICOS signals critically impacted cell fate decisions of Ag-specific CD8+ T cells, resulting in increased frequencies of KLRG-1hiCD127lo cells, altered BLIMP-1, T-bet, and eomesodermin expression, and increased cytolytic capacity as compared with empty vector controls. Interestingly, however, ICOS retrogenic CD8+ T cells also preferentially homed to nonlymphoid organs and exhibited reduced multicytokine functionality and reduced ability to mount secondary recall responses upon challenge in vivo. In sum, our results suggest that an altered differentiation program is induced following early and sustained ICOS expression, resulting in the generation of more cytolyticly potent, terminally differentiated effectors that possess limited capacity for recall response.<\/jats:p>","DOI":"10.4049\/jimmunol.1500218","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T01:49:04Z","timestamp":1451958544000},"page":"1000-1012","source":"Crossref","is-referenced-by-count":8,"title":["Retrogenic ICOS Expression Increases Differentiation of KLRG-1hiCD127loCD8+ T Cells during <i>Listeria<\/i> Infection and Diminishes Recall Responses"],"prefix":"10.1093","volume":"196","author":[{"given":"Danya","family":"Liu","sequence":"first","affiliation":[{"name":"Emory Transplant Center, Emory University , Atlanta, GA 30322"},{"name":"Department of Surgery, Emory University , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen M","family":"Burd","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig M","family":"Coopersmith","sequence":"additional","affiliation":[{"name":"Department of Surgery, Emory University , Atlanta, GA 30322"},{"name":"Emory Critical Care Center, Emory University , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandy L","family":"Ford","sequence":"additional","affiliation":[{"name":"Emory Transplant Center, Emory University , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,2,1]]},"reference":[{"key":"2025030702122722900_r1","doi-asserted-by":"crossref","first-page":"15045","DOI":"10.1073\/pnas.0703767104","article-title":"Increased competition for antigen during priming negatively impacts the generation of memory CD4 T cells","volume":"104","author":"Blair","year":"2007","journal-title":"Proc. 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