{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:41:47Z","timestamp":1790764907787,"version":"4.1.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Atypical hemolytic uremic syndrome (aHUS) is a thrombotic microangiopathy with severe renal injury secondary to an overactive alternative complement pathway (AP). aHUS episodes are often initiated or recur during inflammation. We investigated gene expression of the surface complement regulatory proteins (CD55, CD59, CD46, and CD141 [thrombomodulin]) and AP components in human glomerular microvascular endothelial cells (GMVECs) and in HUVECs, a frequently used investigational model of endothelial cells. Surface complement regulatory proteins were also quantified by flow cytometry. All experiments were done with and without exposure to IL-1\u03b2 or TNF. Without cytokine stimulation, we found that GMVECs had greater AP activation than did HUVECs. With TNF stimulation, THBD gene expression and corresponding CD141 surface presence in HUVECs and GMVECs were reduced, and gene expression of complement components C3 (C3) and factor B (CFB) was increased. Consequently, AP activation, measured by Ba production, was increased, and conversion of protein C (PC) to activated PC by CD141-bound thrombin was decreased, in GMVECs and HUVECs exposed to TNF. IL-1\u03b2 had similar, albeit lesser, effects on HUVEC gene expression, and it only slightly affected GMVEC gene expression. To our knowledge, this is the first detailed study of the expression\/display of AP components and surface regulatory proteins in GMVECs with and without cytokine stimulation. In aHUS patients with an underlying overactive AP, additional stimulation of the AP and inhibition of activated PC\u2013mediated anticoagulation in GMVECs by the inflammatory cytokine TNF are likely to provoke episodes of renal failure.<\/jats:p>","DOI":"10.4049\/jimmunol.1500960","type":"journal-article","created":{"date-parts":[[2015,12,17]],"date-time":"2015-12-17T01:29:32Z","timestamp":1450315772000},"page":"832-845","source":"Crossref","is-referenced-by-count":52,"title":["TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells"],"prefix":"10.1093","volume":"196","author":[{"given":"Sarah E","family":"Sartain","sequence":"first","affiliation":[{"name":"Section of Hematology\u2013Oncology, Department of Pediatrics, Texas Children\u2019s Cancer and Hematology Centers , Houston, TX 77030"},{"name":"Baylor College of Medicine , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy A","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Rice University , Houston, TX 77005"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel L","family":"Moake","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Rice University , Houston, TX 77005"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,1,15]]},"reference":[{"key":"2025030702115558600_r1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1160\/TH07-12-0761","article-title":"Pathological aspects of membranoproliferative glomerulonephritis (MPGN) and haemolytic uraemic syndrome (HUS)\/thrombocytic thrombopenic purpura (TTP)","volume":"101","author":"Benz","year":"2009","journal-title":"Thromb. 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Rare Dis."},{"key":"2025030702115558600_r37","doi-asserted-by":"crossref","first-page":"4199","DOI":"10.1182\/blood-2005-05-2111","article-title":"Hemolytic uremic syndrome-associated Shiga toxins promote endothelial-cell secretion and impair ADAMTS13 cleavage of unusually large von Willebrand factor multimers","volume":"106","author":"Nolasco","year":"2005","journal-title":"Blood"},{"key":"2025030702115558600_r38","first-page":"348","article-title":"Engineering of recombinant soluble CD46: an inhibitor of complement activation","volume":"87","author":"Christiansen","year":"1996","journal-title":"Immunology"},{"key":"2025030702115558600_r39","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.molimm.2009.09.022","article-title":"Hepatitis B virus sensitizes hepatocytes to complement-dependent cytotoxicity through downregulating CD59","volume":"47","author":"Qu","year":"2009","journal-title":"Mol. 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Immunol."},{"key":"2025030702115558600_r41","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0304-3835(97)04737-X","article-title":"TNF-\u03b1 stimulates the biosynthesis of complement C3 and factor B by human umbilical cord vein endothelial cells","volume":"116","author":"Kawakami","year":"1997","journal-title":"Cancer Lett."},{"key":"2025030702115558600_r42","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.thromres.2004.10.010","article-title":"Effects of TNF-\u03b1 and curcumin on the expression of thrombomodulin and endothelial protein C receptor in human endothelial cells","volume":"115","author":"Nan","year":"2005","journal-title":"Thromb. 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Cell. Biol."},{"key":"2025030702115558600_r49","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1084\/jem.177.5.1277","article-title":"Tumor necrosis factor alpha (TNF-alpha)-induced cell adhesion to human endothelial cells is under dominant control of one TNF receptor type, TNF-R55","volume":"177","author":"Mackay","year":"1993","journal-title":"J. Exp. Med."},{"key":"2025030702115558600_r50","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1095\/biolreprod61.4.1017","article-title":"Tumor necrosis factor \u03b1 receptors in microvascular endothelial cells from bovine corpus luteum","volume":"61","author":"Okuda","year":"1999","journal-title":"Biol. Reprod."},{"key":"2025030702115558600_r51","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1160\/TH13-11-0975","article-title":"Role of tumour necrosis factor receptor-1 and nuclear factor-\u03baB in production of TNF-\u03b1-induced pro-inflammatory microparticles in endothelial cells","volume":"112","author":"Lee","year":"2014","journal-title":"Thromb. Haemost."},{"key":"2025030702115558600_r52","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1182\/blood-2004-01-0107","article-title":"Effects of inflammatory cytokines on the release and cleavage of the endothelial cell-derived ultralarge von Willebrand factor multimers under flow","volume":"104","author":"Bernardo","year":"2004","journal-title":"Blood"},{"key":"2025030702115558600_r53","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1172\/JCI117430","article-title":"C5a-induced expression of P-selectin in endothelial cells","volume":"94","author":"Foreman","year":"1994","journal-title":"J. Clin. 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