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U-Omp19 inhibits lysosome cathepsins and APC-derived microsome activity in vitro and partially inhibits lysosomal cathepsin L activity within live APCs. Codelivery of U-Omp19 with the Ag can reduce intracellular Ag digestion and increases Ag half-life in dendritic cells (DCs). U-Omp19 retains the Ag in Lamp-2+ compartments after its internalization and promotes a sustained expression of MHC class I\/peptide complexes in the cell surface of DCs. Consequently, U-Omp19 enhances Ag cross-presentation by DCs to CD8+ T cells. U-Omp19 s.c. delivery induces the recruitment of CD11c+CD8\u03b1+ DCs and monocytes to lymph nodes whereas it partially limits in vivo Ag proteolysis inside DCs. Accordingly, this protein is able to induce CD8+ T cell responses in vivo against codelivered Ag. Antitumor responses were elicited after U-Omp19 coadministration, increasing survival of mice in a murine melanoma challenge model. Collectively, these results indicate that a cysteine protease inhibitor from bacterial origin could be a suitable component of vaccine formulations against tumors.<\/jats:p>","DOI":"10.4049\/jimmunol.1501188","type":"journal-article","created":{"date-parts":[[2016,4,16]],"date-time":"2016-04-16T03:14:31Z","timestamp":1460776471000},"page":"4014-4029","source":"Crossref","is-referenced-by-count":31,"title":["A <i>Brucella<\/i> spp. Protease Inhibitor Limits Antigen Lysosomal Proteolysis, Increases Cross-Presentation, and Enhances CD8+ T Cell Responses"],"prefix":"10.1093","volume":"196","author":[{"given":"Lorena M","family":"Coria","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas\u2013Instituto Tecnol\u00f3gico de Chascom\u00fas, Universidad Nacional de San Mart\u00edn, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas de Argentina , 1650 Buenos Aires 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