{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:52:04Z","timestamp":1791298324522,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Gram-negative bacteria from the Legionella genus are intracellular pathogens that cause a severe form of pneumonia called Legionnaires\u2019 disease. The bacteria replicate intracellularly in macrophages, and the restriction of bacterial replication by these cells is critical for host resistance. The activation of the NAIP5\/NLRC4 inflammasome, which is readily triggered in response to bacterial flagellin, is essential for the restriction of bacterial replication in murine macrophages. Once activated, this inflammasome induces pore formation and pyroptosis and facilitates the restriction of bacterial replication in macrophages. Because investigations related to the NLRC4-mediated restriction of Legionella replication were performed using mice double deficient for caspase-1 and caspase-11, we assessed the participation of caspase-1 and caspase-11 in the functions of the NLRC4 inflammasome and the restriction of Legionella replication in macrophages and in vivo. By using several species of Legionella and mice singly deficient for caspase-1 or caspase-11, we demonstrated that caspase-1 but not caspase-11 was required for pore formation, pyroptosis, and restriction of Legionella replication in macrophages and in vivo. By generating F1 mice in a mixed 129 \u00d7 C57BL\/6 background deficient (129 \u00d7 Casp-11\u2212\/\u2212) or sufficient (129 \u00d7 C57BL\/6) for caspase-11 expression, we found that caspase-11 was dispensable for the restriction of Legionella pneumophila replication in macrophages and in vivo. Thus, although caspase-11 participates in flagellin-independent noncanonical activation of the NLRP3 inflammasome, it is dispensable for the activities of the NLRC4 inflammasome. In contrast, functional caspase-1 is necessary and sufficient to trigger flagellin\/NLRC4-mediated restriction of Legionella spp. infection in macrophages and in vivo.<\/jats:p>","DOI":"10.4049\/jimmunol.1501223","type":"journal-article","created":{"date-parts":[[2015,7,31]],"date-time":"2015-07-31T22:30:11Z","timestamp":1438381811000},"page":"2303-2311","source":"Crossref","is-referenced-by-count":71,"title":["Caspase-1 but Not Caspase-11 Is Required for NLRC4-Mediated Pyroptosis and Restriction of Infection by Flagellated\n                    <i>Legionella<\/i>\n                    Species in Mouse Macrophages and In Vivo"],"prefix":"10.1093","volume":"195","author":[{"given":"Daiane M","family":"Cerqueira","sequence":"first","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, S\u00e3o Paulo 14049-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo S F","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, S\u00e3o Paulo 14049-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre L N","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, S\u00e3o Paulo 14049-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa D","family":"Cunha","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, S\u00e3o Paulo 14049-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario S","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, S\u00e3o Paulo 14049-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,9,1]]},"reference":[{"key":"2025030704085811600_r1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1128\/CMR.00052-09","article-title":"Molecular pathogenesis of infections caused by Legionella pneumophila","volume":"23","author":"Newton","year":"2010","journal-title":"Clin. 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