{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T14:30:03Z","timestamp":1775313003773,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Localization of the platelet glycoprotein Ib\u2013IX complex to the membrane lipid domain is essential for platelet adhesion to von Willebrand factor and subsequent platelet activation in vitro. Yet, the in vivo importance of this localization has never been addressed. We recently found that the disulfide linkage between Ib\u03b1 and Ib\u03b2 is critical for the association of Ib\u03b1 with the glycosphingolipid-enriched membrane domain; in this study, we established a transgenic mouse model expressing this mutant human Ib\u03b1 that is also devoid of endogenous Ib\u03b1 (H\u03b1SSM\u03b1\u2212\/\u2212). Characterization of this model demonstrated a similar dissociation of Ib\u03b1 from murine platelet glycosphingolipid-enriched membrane to that expressed in Chinese hamster ovary cells, which correlates well with the impaired adhesion of the transgenic platelets to von Willebrand factor ex vivo and in vivo. Furthermore, we bred our transgenic mice into an atherosclerosis-prone background (H\u03b1SSM\u03b1\u2212\/\u2212ApoE\u2212\/\u2212 and H\u03b1WTM\u03b1\u2212\/\u2212ApoE\u2212\/\u2212). We observed that atheroma formation was significantly inhibited in mutant mice where fewer platelet-bound CD11c+ leukocytes were circulating (CD45+\/CD11c+\/CD41+) and residing in atherosclerotic lesions (CD45+\/CD11c+), suggesting that platelet-mediated adhesion and infiltration of CD11c+ leukocytes may be one of the mechanisms. To our knowledge, these observations provide the first in vivo evidence showing that the membrane GEM is physiologically and pathophysiologically critical in the function of the glycoprotein Ib\u2013IX complex.<\/jats:p>","DOI":"10.4049\/jimmunol.1501946","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T03:07:24Z","timestamp":1463972844000},"page":"288-295","source":"Crossref","is-referenced-by-count":7,"title":["Defective Association of the Platelet Glycoprotein Ib\u2013IX Complex with the Glycosphingolipid-Enriched Membrane Domain Inhibits Murine Thrombus and Atheroma Formation"],"prefix":"10.1093","volume":"197","author":[{"given":"Hao","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Hospital Infection Management of Nanfang Hospital, Southern Medical University , Guangzhou 510515,","place":["China"]},{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1871-7264","authenticated-orcid":false,"given":"Yali","family":"Ran","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Da","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanner S","family":"Shaw","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4585-2744","authenticated-orcid":false,"given":"Dan","family":"Shang","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"},{"name":"Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anilkumar K","family":"Reddy","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Puget Sound Blood Center, Division of Hematology, Department of Medicine, University of Washington , Seattle, WA 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christie M","family":"Ballantyne","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry","family":"Ware","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Arkansas for Medical Sciences , Little Rock, AR 72205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaizhu","family":"Wu","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0359-0029","authenticated-orcid":false,"given":"Yuandong","family":"Peng","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine , Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,7,1]]},"reference":[{"key":"2025030617452024900_r1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1182\/blood-2006-05-024091","article-title":"Glycoprotein Ibalpha forms disulfide bonds with 2 glycoprotein Ibbeta subunits in the resting platelet","volume":"109","author":"Luo","year":"2007","journal-title":"Blood"},{"key":"2025030617452024900_r2","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1111\/j.1432-1033.1985.tb09152.x","article-title":"Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane","volume":"151","author":"Berndt","year":"1985","journal-title":"Eur. 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