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We report in this study that i.v. delivery of rhabdoviral vectors leads to direct infection of follicular B cells in the spleen, where the earliest evidence of secondary T cell responses was observed. This allows booster immunizations to rapidly expand CD8+ central memory T cells (TCM) during the acute phase of the primary response that is dominated by TEFF. Interestingly, although the ablation of B cells before boosting with rhabdoviral vectors diminishes the expansion of memory T cells, B cells do not present Ags directly. Instead, depletion of CD11c+ dendritic cells abrogates secondary T cell expansion, suggesting that virus-infected follicular B cells may function as an Ag source for local DCs to subsequently capture and present the Ag. Because TCM are located within B cell follicles in the spleen whereas TEFF cannot traffic through follicular regions, Ag production and presentation by follicular APCs represent a unique mechanism to secure engagement of TCM during an ongoing effector response. Our data offer insights into novel strategies for rapid expansion of CD8+ T cells using prime-boost vaccines by targeting privileged sites for Ag presentation.<\/jats:p>","DOI":"10.4049\/jimmunol.1600106","type":"journal-article","created":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T16:38:59Z","timestamp":1461861539000},"page":"4587-4595","source":"Crossref","is-referenced-by-count":43,"title":["Privileged Antigen Presentation in Splenic B Cell Follicles Maximizes T Cell Responses in Prime-Boost Vaccination"],"prefix":"10.1093","volume":"196","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8335-1118","authenticated-orcid":false,"given":"Byram W","family":"Bridle","sequence":"first","affiliation":[{"name":"Department of Pathobiology, Ontario Veterinary College, University of Guelph , Guelph, Ontario N1G 2W1 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department 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,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2836-5813","authenticated-orcid":false,"given":"Sandeep","family":"Koshy","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University , Hamilton, Ontario L8N 3Z5 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek","family":"Clouthier","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University , Hamilton, Ontario L8N 3Z5 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University , Hamilton, Ontario L8N 3Z5 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8355-7562","authenticated-orcid":false,"given":"Jonathan","family":"Pol","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University , Hamilton, Ontario L8N 3Z5 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8545-8322","authenticated-orcid":false,"given":"Stephanie L","family":"Swift","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University , Hamilton, Ontario L8N 3Z5 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn M E","family":"Bowdish","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, 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