{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:45:45Z","timestamp":1790142345901,"version":"4.0.1"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Follicular lymphoma (FL) is the most common form of indolent non-Hodgkin lymphoma. This malignancy is considered virtually incurable, with high response rates to therapy but frequent relapses. We investigated the ability of monocyte-derived dendritic cells generated in the presence of IFN-\u03b1 and GM-CSF (IFN-DC) and loaded with apoptotic lymphoma cells to activate immune responses against FL cells, with the ultimate goal of designing novel patient-specific vaccination strategies for the treatment of FL. In this article, we show that apoptotic tumor cell\u2013loaded IFN-DC from FL patients, which were cultured for 2 wk with autologous lymphocytes, led to Th1 response skewing, based on significantly higher levels of IFN-\u03b3 production and a remarkable increase in CD8+ and NK cell frequency, consistent with the detection of enhanced cytotoxic effector function toward autologous FL cells. IFN-DC were found to promote efficient NK cell activation, increased expression of cytotoxicity receptors, and extensive IFN-\u03b3 production in the virtual absence of IL-10. Moreover, direct recognition and killing of primary autologous lymphoma cells by activated NK cells from FL patients was also demonstrated. A critical role was demonstrated for MHC class I\u2013related chain A and B and membrane-bound IL-15 in IFN-DC\u2013mediated NK cell activation and early IFN-\u03b3 production. The overall results indicate that IFN-DC loaded with autologous apoptotic FL cells represent a valuable tool for improving the potency of therapeutic cancer vaccines through the efficient induction of NK cell activation and promotion of CD8+ T cell antitumor immunity.<\/jats:p>","DOI":"10.4049\/jimmunol.1600262","type":"journal-article","created":{"date-parts":[[2016,6,30]],"date-time":"2016-06-30T03:03:49Z","timestamp":1467255829000},"page":"795-806","source":"Crossref","is-referenced-by-count":23,"title":["NK Cell Activation in the Antitumor Response Induced by IFN-\u03b1 Dendritic Cells Loaded with Apoptotic Cells from Follicular Lymphoma Patients"],"prefix":"10.1093","volume":"197","author":[{"given":"Caterina","family":"Lapenta","sequence":"first","affiliation":[{"name":"Reparto di Immunoterapia Sperimentale, Dipartimento di Ematologia, Oncologia e Medicina Sperimentale, Istituto Superiore di Sanit\u00e0 , 00161 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Donati","sequence":"additional","affiliation":[{"name":"Reparto di Immunoterapia Sperimentale, Dipartimento di Ematologia, Oncologia e Medicina Sperimentale, Istituto Superiore di Sanit\u00e0 , 00161 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Spadaro","sequence":"additional","affiliation":[{"name":"Reparto di Immunoterapia Sperimentale, Dipartimento di Ematologia, Oncologia e Medicina Sperimentale, Istituto Superiore di Sanit\u00e0 , 00161 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Castaldo","sequence":"additional","affiliation":[{"name":"Unit\u00e0 di Chirurgia Generale, Azienda Ospedaliera Sant\u2019Andrea, Universit\u00e0 La Sapienza , 00189 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Belardelli","sequence":"additional","affiliation":[{"name":"Reparto di Immunoterapia Sperimentale, Dipartimento di Ematologia, Oncologia e Medicina Sperimentale, Istituto Superiore di Sanit\u00e0 , 00161 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C","family":"Cox","sequence":"additional","affiliation":[{"name":"Unit\u00e0 di Ematologia, Azienda Ospedaliera Sant\u2019Andrea, Universit\u00e0 La Sapienza , 00189 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano M","family":"Santini","sequence":"additional","affiliation":[{"name":"Reparto di Immunoterapia Sperimentale, Dipartimento di Ematologia, Oncologia e Medicina Sperimentale, Istituto Superiore di Sanit\u00e0 , 00161 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,8,1]]},"reference":[{"key":"2025030617470081600_r1","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1182\/blood.V89.11.3909","article-title":"A clinical evaluation of the International Lymphoma Study Group classification of non-Hodgkin\u2019s lymphoma","volume":"89","author":"The Non-Hodgkin\u2019s Lymphoma Classification Project","year":"1997","journal-title":"Blood"},{"key":"2025030617470081600_r2","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1056\/NEJM198412063112303","article-title":"The natural history of initially untreated low-grade non-Hodgkin\u2019s lymphomas","volume":"311","author":"Horning","year":"1984","journal-title":"N. 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