{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:37:49Z","timestamp":1774960669594,"version":"3.50.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Thymic stromal lymphopoietin (TSLP) and IL-7 are related cytokines that mediate growth and differentiation events in the immune system. They signal through IL-7R\u03b1\u2013containing receptors. Target cells of TSLP in Th2 responses include CD4 T cells and dendritic cells (DCs). Although it has been reported that expression of TSLP receptor (TSLPR) on CD4 T cells is required for OVA-induced lung inflammation, DCs have also been shown to be target cells of TSLP. In this study, we show that murine ex vivo splenic DCs are unresponsive to TSLP, as they fail to phosphorylate STAT5, but in vitro overnight culture, especially in presence of IL-4, renders DCs responsive to both TSLP and IL-7. This induced responsiveness is accompanied by dramatic upregulation of IL-7R\u03b1 on DCs with little change in expression of TSLPR or of \u03b3c. In splenic DCs, the induction of IL-7R\u03b1 occurs mainly in CD8\u2212 DCs. In vivo, we found that IL-4 has a differential regulatory role on expression of IL-7R\u03b1 depending on the cell type; IL-4 decreases IL-7R\u03b1 expression on CD4 T cells whereas it upregulates the expression on DCs. Our results indicate that the induction of IL-7R\u03b1 expression on DCs is critical for TSLP responsiveness and that IL-4 can upregulate IL-7R\u03b1 on DCs.<\/jats:p>","DOI":"10.4049\/jimmunol.1600753","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T00:40:15Z","timestamp":1492044015000},"page":"3909-3918","source":"Crossref","is-referenced-by-count":12,"title":["IL-7R\u03b1 Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin"],"prefix":"10.1093","volume":"198","author":[{"given":"Laura","family":"Kummola","sequence":"first","affiliation":[{"name":"Faculty of Medicine and Life Sciences, University of Tampere , 33520 Tampere,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsanna","family":"Ortutay","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Life Sciences, University of Tampere , 33520 Tampere,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephane","family":"Caucheteux","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanna","family":"H\u00e4m\u00e4l\u00e4inen","sequence":"additional","affiliation":[{"name":"Biomeditech, University of Tampere , 33014 Tampere,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saara","family":"Aittom\u00e4ki","sequence":"additional","affiliation":[{"name":"Biomeditech, University of Tampere , 33014 Tampere,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7621-2753","authenticated-orcid":false,"given":"Ryoji","family":"Yagi","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinfang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7541-3921","authenticated-orcid":false,"given":"Marko","family":"Pesu","sequence":"additional","affiliation":[{"name":"Biomeditech, University of Tampere , 33014 Tampere,","place":["Finland"]},{"name":"Department of Dermatology, Tampere University Hospital , 33521 Tampere,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E","family":"Paul","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilkka S","family":"Junttila","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Life Sciences, University of Tampere , 33520 Tampere,","place":["Finland"]},{"name":"Fimlab Laboratories , 33520 Tampere,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,5,15]]},"reference":[{"key":"2025030615125380300_r1","doi-asserted-by":"crossref","first-page":"19455","DOI":"10.1073\/pnas.1008271107","article-title":"Thymic stromal lymphopoietin-mediated STAT5 phosphorylation via kinases JAK1 and JAK2 reveals a key difference from IL-7\u2013induced signaling","volume":"107","author":"Rochman","year":"2010","journal-title":"Proc. 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