{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:40:51Z","timestamp":1773211251986,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Initial studies associated the HLA class I A*01 and B*08 alleles with celiac disease (CD) susceptibility. Subsequent analyses showed a primary association with HLA class II alleles encoding for the HLA DQ2.5 molecule. Because of the strong linkage disequilibrium of A*01 and B*08 alleles with the DR3-DQ2.5 haplotype and a recent genome-wide association study indicating that B*08 and B*39 are predisposing genes, the etiologic role of HLA class I in CD pathogenesis needs to be addressed. We screened gliadin proteins (2\u03b1-, 2\u03c9-, and 2\u03b3-gliadin) using bioinformatic algorithms for the presence of peptides predicted to bind A*0101 and B*0801 molecules. The top 1% scoring 9- and 10-mer peptides (N = 97, total) were synthesized and tested in binding assays using purified A*0101 and B*0801 molecules. Twenty of ninety-seven peptides bound B*0801 and only 3 of 97 bound A*0101 with high affinity (IC50 &amp;lt; 500 nM). These 23 gliadin peptides were next assayed by IFN-\u03b3 ELISPOT for recognition in peripheral blood cells of CD patients and healthy controls carrying the A*0101 and\/or B*0801 genes and in A*0101\/B*0801\u2212 CD patients. Ten of the twenty-three peptides assayed recalled IFN-\u03b3 responses mediated by CD8+ T cells in A*0101\/B*0801+ patients with CD. Two peptides were restricted by A*0101, and eight were restricted by B*0801. Of note, 50% (5\/10) of CD8+ T cell epitopes mapped within the \u03b3-gliadins. Our results highlight the value of predicted binding to HLA molecules for identifying gliadin epitopes and demonstrate that HLA class I molecules restrict the anti-gluten T cell response in CD patients.<\/jats:p>","DOI":"10.4049\/jimmunol.1601208","type":"journal-article","created":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T01:50:18Z","timestamp":1486000218000},"page":"1838-1845","source":"Crossref","is-referenced-by-count":15,"title":["Gliadin-Specific CD8+ T Cell Responses Restricted by HLA Class I A*0101 and B*0801 Molecules in Celiac Disease Patients"],"prefix":"10.1093","volume":"198","author":[{"given":"Stefania","family":"Picascia","sequence":"first","affiliation":[{"name":"Institute of Protein Biochemistry\u2013National Research Council , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Sidney","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology , La Jolla, CA 92130"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Camarca","sequence":"additional","affiliation":[{"name":"Institute of Food Sciences\u2013National Research Council , 83100 Avellino,","place":["Italy"]},{"name":"Department of Translational Medical Science and European Laboratory for Investigation and Food-Induced Diseases, University Federico II , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7294-8277","authenticated-orcid":false,"given":"Giuseppe","family":"Mazzarella","sequence":"additional","affiliation":[{"name":"Institute of Food Sciences\u2013National Research Council , 83100 Avellino,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Giardullo","sequence":"additional","affiliation":[{"name":"Gastroenterology Unit, Hospital Moscati , 83100 Avellino,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Greco","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Science and European Laboratory for Investigation and Food-Induced Diseases, University Federico II , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Auricchio","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Science and European Laboratory for Investigation and Food-Induced Diseases, University Federico II , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Auricchio","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Science and European Laboratory for Investigation and Food-Induced Diseases, University Federico II , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Troncone","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Science and European Laboratory for Investigation and Food-Induced Diseases, University Federico II , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7013-2250","authenticated-orcid":false,"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology , La Jolla, CA 92130"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Gianfrani","sequence":"additional","affiliation":[{"name":"Institute of Protein Biochemistry\u2013National Research Council , 80131 Naples,","place":["Italy"]},{"name":"Department of Translational Medical Science and European Laboratory for Investigation and Food-Induced Diseases, University Federico II , 80131 Naples,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,3,1]]},"reference":[{"key":"2025031312200587300_r1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1146\/annurev.immunol.18.1.53","article-title":"Molecular basis of celiac disease","volume":"18","author":"Sollid","year":"2000","journal-title":"Annu. 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