{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:06:02Z","timestamp":1790849162642,"version":"4.1.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Single Ag-specific CD8+ T cells from IFN-\u03b3-deficient (GKO) or perforin-deficient (PKO) mice provide substantial immunity against murine infection with Listeria monocytogenes. To address the potential for redundancy between perforin and IFN-\u03b3 as CD8+ T cell effector mechanisms, we generated perforin\/IFN-\u03b3 (PKO\/GKO) double-deficient mice. PKO\/GKO-derived CD8+ T cells specific for the immunodominant listeriolysin O (LLO91\u201399) epitope provide immunity to LM infection similar to that provided by Ag-matched wild-type (WT) CD8+ T cells in the liver but reduced in the spleen. Strikingly, polyclonal CD8+ T cells from immunized PKO\/GKO mice were \u223c100-fold more potent in reducing bacterial numbers than the same number of polyclonal CD8+ T cells from immunized WT mice. This result is probably quantitative, because the frequency of the CD8+ T cell response against the immunodominant LLO91\u201399 epitope is &amp;gt;4.5-fold higher in PKO\/GKO mice than WT mice at 7 days after identical immunizations. Moreover, PKO\/GKO mice can be immunized by a single infection with attenuated Listeria to resist &amp;gt;80,000-fold higher challenges with virulent organisms than naive PKO\/GKO mice. These data demonstrate that neither perforin nor IFN-\u03b3 is required for the development or expression of adaptive immunity to LM. In addition, the results suggest the potential for perforin and IFN-\u03b3 to regulate the magnitude of the CD8+ T cell response to infection.<\/jats:p>","DOI":"10.4049\/jimmunol.164.12.6444","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:59:29Z","timestamp":1398031169000},"page":"6444-6452","source":"Crossref","is-referenced-by-count":62,"title":["Adaptive Immunity and Enhanced CD8+ T Cell Response to\n                    <i>Listeria monocytogenes<\/i>\n                    in the Absence of Perforin and IFN-\u03b3"],"prefix":"10.1093","volume":"164","author":[{"given":"Vladimir P","family":"Badovinac","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John T","family":"Harty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"},{"name":"Interdisciplinary Graduate Program in Immunology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,6,15]]},"reference":[{"key":"2026020214034014600_R1","first-page":"89","volume-title":"Curr. 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