{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:10:56Z","timestamp":1791047456726,"version":"4.1.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Macrophage inflammatory protein (MIP-1\u03b1), a member of the CC chemokine subfamily, has been shown to attract T cells and monocytes in vitro and to be expressed at sites of inflammation. Although the in vitro activities of MIP-1\u03b1 have been well documented, the in vivo biological activities of MIP-1\u03b1 in humans have not been studied. To address this, we challenged human subjects by intradermal injection with up to 1000 pmol of MIP-1\u03b1 and performed biopsies 2, 10, and 24 h later. Although no acute cutaneous or systemic reactions were noted, endothelial cell activation, as indicated by the expression of E-selectin, was observed. In agreement with its in vitro activity, monocyte, lymphocyte, and, to a lesser degree, eosinophil infiltration was observed, peaking at 10\u201324 h. Surprisingly, in contrast to its reported lack of in vitro neutrophil-stimulating activity, a rapid infiltration of neutrophils was observed in vivo. This neutrophil infiltration occurred as early as 2 h, preceding the appearance of other cells, and peaked at 10 h. Interestingly, we found that neutrophils in whole blood, but not after isolation, expressed CCR1 on their cell surface. This CCR1 was thought to be functional as assessed by neutrophil CD11b up-regulation following whole-blood MIP-1\u03b1 stimulation. These studies substantiate the biological effects of MIP-1\u03b1 on monocytes and lymphocytes and uncover the previously unrecognized activity of MIP-1\u03b1 to induce neutrophil infiltration and endothelial cell activation, underscoring the need to evaluate chemokines in vivo in humans.<\/jats:p>","DOI":"10.4049\/jimmunol.164.6.3392","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T20:28:33Z","timestamp":1398198513000},"page":"3392-3401","source":"Crossref","is-referenced-by-count":85,"title":["Cutaneous Injection of Human Subjects with Macrophage Inflammatory Protein-1\u03b1 Induces Significant Recruitment of Neutrophils and Monocytes"],"prefix":"10.1093","volume":"164","author":[{"given":"Sang Chin","family":"Lee","sequence":"first","affiliation":[{"name":"Catholic University School of Medicine , Seoul ,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E","family":"Brummet","sequence":"additional","affiliation":[{"name":"Johns Hopkins Asthma and Allergy Center , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed","family":"Shahabuddin","sequence":"additional","affiliation":[{"name":"Johns Hopkins Asthma and Allergy Center , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thasia G","family":"Woodworth","sequence":"additional","affiliation":[{"name":"Pfizer, Inc. , Groton, CT 06340"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve N","family":"Georas","sequence":"additional","affiliation":[{"name":"Johns Hopkins Asthma and Allergy Center , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin M","family":"Leiferman","sequence":"additional","affiliation":[{"name":"Mayo Clinic , Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven C","family":"Gilman","sequence":"additional","affiliation":[{"name":"Pfizer, Inc. , Groton, CT 06340"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana","family":"Stellato","sequence":"additional","affiliation":[{"name":"Johns Hopkins Asthma and Allergy Center , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron P","family":"Gladue","sequence":"additional","affiliation":[{"name":"Pfizer, Inc. , Groton, CT 06340"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P","family":"Schleimer","sequence":"additional","affiliation":[{"name":"Johns Hopkins Asthma and Allergy Center , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A","family":"Beck","sequence":"additional","affiliation":[{"name":"Johns Hopkins Asthma and Allergy Center , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,3,15]]},"reference":[{"key":"2025032908422031500_R1","first-page":"R384","volume-title":"Curr. 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