{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T22:27:15Z","timestamp":1750112835069,"version":"3.40.3"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The maturation of IgM-expressing B cells to IgM-secreting plasma cells is associated with both an increase in \u03bc mRNA and the ratio of secreted to membrane forms of \u03bc mRNA which differ at the 3\u2032 termini. In contrast, both in vitro and in vivo the secreted form of \u03b1 mRNA is predominant at all stages in the development of a secretory IgA response. Previous studies demonstrated that preferential usage of the \u03b1s poly(A) site does not result from transcription termination and is independent of either the poly(A) sites or the 3\u2032 splice site associated with the exon encoding the membrane exon of IgA (\u03b1M). The present study demonstrates that a 349-bp region located 774 bp 3\u2032 to the \u03b1s poly(A) site is required for the preferential usage of the \u03b1s terminus. This region, which is the first isotype-specific cis-acting regulatory sequence not immediately adjacent to a secretory poly(A) site to be identified, contains regulatory elements that increase the efficiency of polyadenylation\/cleavage. A ubiquitous, \u223c58-kDa RNA-binding protein interacts specifically with this regulatory region. These studies support the premise that cis-acting elements unique to each CH gene can impinge upon a common mechanism regulating Ig mRNA processing.<\/jats:p>","DOI":"10.4049\/jimmunol.164.7.3659","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:24:59Z","timestamp":1398032699000},"page":"3659-3665","source":"Crossref","is-referenced-by-count":8,"title":["Correct Immunoglobulin \u03b1 mRNA Processing Depends on Specific Sequence in the C\u03b13-\u03b1M Intron"],"prefix":"10.1093","volume":"164","author":[{"given":"John H","family":"Coyle","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Virginia Commonwealth University , Richmond, VA 23298"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah A","family":"Lebman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Virginia Commonwealth University , Richmond, VA 23298"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,4,1]]},"reference":[{"volume-title":"J. 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