{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:28:11Z","timestamp":1790166491142,"version":"4.1.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Inhibition of class II trans-activator (CIITA) expression prevents embryonic trophoblast cells from up-regulating MHC class II genes in response to IFN-\u03b3. This is thought to be one mechanism of maternal tolerance to the fetal allograft. The CIITA gene is regulated by four distinct promoters; promoter III directs constitutive (B cell) expression, and promoter IV regulates IFN-\u03b3-inducible expression. Using in vivo genomic footprinting, promoter-reporter analysis, Southern blot analysis, and RT-PCR, we have examined the cause of CIITA silencing in a trophoblast-derived cell line. We report here that methylation of promoter IV DNA at CpG sites in Jar cells prevents promoter occupancy and IFN-\u03b3-inducible transcription. The inhibition of CpG methylation in Jar cells by treatment with 5-aza-2\u2032-deoxycytidine restores IFN-\u03b3 inducibility to CIITA. This is the first description of an epigenetic mechanism involved in regulation of CIITA and MHC class II gene expression.<\/jats:p>","DOI":"10.4049\/jimmunol.164.8.4143","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T02:13:19Z","timestamp":1398132799000},"page":"4143-4149","source":"Crossref","is-referenced-by-count":71,"title":["Methylation of Class II <i>trans<\/i>-Activator Promoter IV: A Novel Mechanism of MHC Class II Gene Control"],"prefix":"10.1093","volume":"164","author":[{"given":"Ann C","family":"Morris","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendi E","family":"Spangler","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy M","family":"Boss","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,4,15]]},"reference":[{"key":"2025032908472441900_R1","first-page":"991","volume-title":"Annu. 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