{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:29:46Z","timestamp":1771003786810,"version":"3.50.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A new paradigm for designing vaccines against certain microbial pathogens, including Chlamydia trachomatis, is based on the induction of local mucosal Th1 response. IL-10 is an anti-inflammatory cytokine that exerts negative immunoregulatory influence on Th1 response. This study investigated whether biochemical modulation of endogenous IL-10 expression at the level of APCs is a practical strategy for enhancing the specific Th1 response against pathogens controlled by Th1 immunity. The results revealed that the high resistance of genetically engineered IL-10\u2212\/\u2212 (IL-10KO) mice to genital chlamydial infection is a function of the predilection of their APCs to rapidly and preferentially activate a high Th1 response. Thus, in microbiological analysis, IL-10KO mice suffered a shorter duration of infection, less microbial burden, and limited ascending infection than immunocompetent wild-type mice. Also, IL-10KO were resistant to reinfection after 8 wk of the primary infection. Cellular and molecular immunologic evaluation indicated that IL-10KO mice induced greater frequency of chlamydial-specific Th1 response following C. trachomatis infection. Moreover, IL-10KO APCs or antisense IL-10 oligonucleotide-treated wild-type APCs were potent activators of Th1 response from naive or immune T cells. Furthermore, both Ag-pulsed dendritic cells from IL-10KO mice and IL-10 antisense-treated dendritic cells from wild-type mice were efficient cellular vaccines in adoptive immunotherapeutic vaccination against genital chlamydial infection. These findings may furnish a novel immunotherapeutic strategy for boosting the Th1 response against T cell-controlled pathogens and tumors, using IL-10-deficient APCs as vaccine delivery agents.<\/jats:p>","DOI":"10.4049\/jimmunol.164.8.4212","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:13:19Z","timestamp":1398118399000},"page":"4212-4219","source":"Crossref","is-referenced-by-count":106,"title":["Suppression of Endogenous IL-10 Gene Expression in Dendritic Cells Enhances Antigen Presentation for Specific Th1 Induction: Potential for Cellular Vaccine Development"],"prefix":"10.1093","volume":"164","author":[{"given":"Joseph U","family":"Igietseme","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Morehouse School of Medicine , Atlanta, GA 30310"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Godwin A","family":"Ananaba","sequence":"additional","affiliation":[{"name":"Department of Biology, Spelman College , Atlanta, GA 30314"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Bolier","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Morehouse School of Medicine , Atlanta, GA 30310"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samera","family":"Bowers","sequence":"additional","affiliation":[{"name":"Department of Biology, Spelman College , Atlanta, GA 30314"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terri","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Morehouse School of Medicine , Atlanta, GA 30310"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tesfaye","family":"Belay","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Morehouse School of Medicine , Atlanta, GA 30310"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis O","family":"Eko","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Morehouse School of Medicine , Atlanta, GA 30310"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah","family":"Lyn","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Morehouse School of Medicine , Atlanta, GA 30310"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn M","family":"Black","sequence":"additional","affiliation":[{"name":"National Center for Infectious Diseases, Centers for Disease Control and Prevention , Atlanta, GA 30333"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,4,15]]},"reference":[{"key":"2026020213180727600_R1"},{"key":"2026020213180727600_R2","first-page":"3","volume-title":"Chlamydial Infections","year":"1998"},{"key":"2026020213180727600_R3"},{"key":"2026020213180727600_R4","first-page":"111","volume-title":"J. 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