{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:42:12Z","timestamp":1790937732685,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The prevalence of type I allergy to Hevea brasiliensis latex is particularly high among individuals with frequent exposure such as health care workers and patients with spina bifida (SB). Due to a birth defect of the spinal canal and the resulting neurological and orthopedic defects, these patients require multiple surgeries during childhood. SB patients display a unique pattern of sensitization: IgE-reactivity is preferentially directed against Hev b 3 and Hev b 1, two latex allergens with high sequence similarity. In this study, we analyzed the T cell response to Hev b 3 in latex-allergic SB patients using poly-, oligo-, and monoclonal T lymphocyte cultures. All T cell clones (TCC) were CD3\/CD4-positive and expressed the \u03b1\u03b2 TCR. According to their cytokine production pattern (IL-4 vs IFN-\u03b3), 12 of 21 TCC were classified as Th2-like, 2 of 21 were Th1-like, and 7 of 21 belonged to a Th0-like subset. Using 11 T cell lines and 21 TCC, nine T cell stimulating fragments were determined out of 52 overlapping 12-mer peptides representing the complete amino acid sequence of Hev b 3. Ag presentation of one dominant T cell epitope could be associated with a four-amino acid binding motif (YSTS, position 11\u201313) in the \u03b21 chain of HLA-DR molecules expressed by the respective patients. No reactivity was observed when Hev b 3-reactive T cell lines or TCC were incubated with peptides representing homologous parts of the Hev b 1 molecule, i.e., no cross-reactivity between Hev b 3 and Hev b 1 at the T cell level was evident.<\/jats:p>","DOI":"10.4049\/jimmunol.164.8.4393","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:13:19Z","timestamp":1398118399000},"page":"4393-4398","source":"Crossref","is-referenced-by-count":34,"title":["Characterization of T Cell Responses to Hev b 3, an Allergen Associated with Latex Allergy in Spina Bifida Patients"],"prefix":"10.1093","volume":"164","author":[{"given":"Barbara","family":"Bohle","sequence":"first","affiliation":[{"name":"Department of General and Experimental Pathology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of General and Experimental Pathology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Vollmann","sequence":"additional","affiliation":[{"name":"Department of General and Experimental Pathology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietke","family":"Buck","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital, Virchow Clinic, Humboldt University , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bodo","family":"Niggemann","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital, Virchow Clinic, Humboldt University , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Sze\u0301pfalusi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gottfried","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Blood Group Serology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otto","family":"Scheiner","sequence":"additional","affiliation":[{"name":"Department of General and Experimental Pathology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heimo","family":"Breiteneder","sequence":"additional","affiliation":[{"name":"Department of General and Experimental Pathology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"Ebner","sequence":"additional","affiliation":[{"name":"Department of General and Experimental Pathology, University of Vienna , Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,4,15]]},"reference":[{"key":"2026020213173280700_R1","first-page":"319","volume-title":"J. 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