{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T08:03:12Z","timestamp":1778659392957,"version":"3.51.4"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The mucosa of the conjunctiva is an important site of entry for environmental Ags as well as Ags emanating from the eye itself. However, very little is known about T cell recognition of Ag introduced through this important mucosal site. We have characterized the in vivo process of CD4 T cell recognition of Ag delivered via the conjunctival mucosa. Application of soluble OVA to the conjunctiva of BALB\/c mice induced potent T cell tolerance. APC-presenting OVA peptide in vivo was only found in the submandibular lymph node and not in other lymph nodes, spleen, or nasal-associated lymphoid tissue. Similarly, in TCR transgenic DO11.10 adoptive transfer mice, OVA-specific CD4+ T cell clonal expansion was only observed in the submandibular lymph node following conjunctival application of peptide. These experiments thus define a highly specific lymphatic drainage pathway from the conjunctiva. OVA-specific T cell clonal expansion peaked at day 3 following initiation of daily OVA administration and gradually declined during the 10-day treatment period, but remained elevated compared with nontreated adoptive transfer mice. During this period, the T cells expressed activation markers, and proliferated and secreted IL-2 in vitro in response to OVA stimulation. In contrast, these cells were unable to clonally expand in vivo, or proliferate in vitro following a subsequent OVA\/CFA immunization. These results suggest that Ag applied to a mucosal site can be efficiently presented in a local draining lymph node, resulting in initial T cell priming and clonal expansion, followed by T cell anergy.<\/jats:p>","DOI":"10.4049\/jimmunol.164.9.4543","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:34:50Z","timestamp":1398119690000},"page":"4543-4550","source":"Crossref","is-referenced-by-count":44,"title":["In Vivo Behavior of Peptide-Specific T Cells During Mucosal Tolerance Induction: Antigen Introduced Through the Mucosa of the Conjunctiva Elicits Prolonged Antigen-Specific T Cell Priming Followed by Anergy"],"prefix":"10.1093","volume":"164","author":[{"given":"Rita M","family":"Egan","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Kentucky Medical Center , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Yorkey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Kentucky Medical Center , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Black","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Kentucky Medical Center , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai Khan","family":"Loh","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Kentucky Medical Center , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia L","family":"Stevens","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Kentucky Medical Center , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene","family":"Storozynsky","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, University of Rochester Cancer Center , Rochester, NY 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edith M","family":"Lord","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, University of Rochester Cancer Center , Rochester, NY 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G","family":"Frelinger","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, University of Rochester Cancer Center , Rochester, NY 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerold G","family":"Woodward","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Kentucky Medical Center , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,5,1]]},"reference":[{"key":"2026020213184913800_R1","first-page":"585","volume-title":"Ophthalmology","year":"1983"},{"key":"2026020213184913800_R2","first-page":"181","volume-title":"Invest. 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