{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:52:28Z","timestamp":1789390348614,"version":"build-2803163510"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The gene LamC2 encoding the \u03b32 chain of laminin 5, an epithelial cell-specific extracellular matrix protein, was identified in a PCR-based subtracted cDNA library from mouse thymic stromal cells. The mRNA existed in two alternative forms (5.1 and 2.4 kb). The full-length message was highly expressed in SCID thymus and in a nurse cell line, but not in other thymic epithelial cell lines, while the short form was more widely expressed. In situ hybridization and immunohistochemical staining revealed laminin 5 expression mostly in the subcapsular region of the adult thymus. Addition to fetal thymic organ cultures of a cell adhesion-blocking mAb to the \u03b13 chain of laminin 5 interrupted T cell development. There was a 40% reduction in the total yield of thymocytes, and the most profound decrease (75\u201390%) was seen in the CD25+CD44+ and CD25+CD44\u2212subsets of the CD4\u2212CD8\u2212 double negative fraction. Most of the surviving double negative thymocytes expressed Sca-1, and there were significant increases in the number of cells with CD69 expression and in the fraction of annexin V-stained cells. None of these changes were observed with a nonblocking anti-laminin \u03b13 chain mAb. These results suggest that the interaction between double negative thymoctyes and laminin 5 made by subcapsular epithelial cells is required for the survival and differentiation of mouse thymocytes.<\/jats:p>","DOI":"10.4049\/jimmunol.165.1.192","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:07:57Z","timestamp":1398038877000},"page":"192-201","source":"Crossref","is-referenced-by-count":38,"title":["Epithelial Cell-Specific Laminin 5 Is Required for Survival of Early Thymocytes"],"prefix":"10.1093","volume":"165","author":[{"given":"Moon Gyo","family":"Kim","sequence":"first","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"},{"name":"National Creative Research Initiative Center for Genetic Reprograming, Seoul National University , Seoul ,","place":["Korea"]},{"name":"Institute for Molecular Biology and Genetics, Seoul National University , Seoul ,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwanghee","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biology and Genetics, Seoul National University , Seoul ,","place":["Korea"]},{"name":"Department of Molecular Biology, Seoul National University , Seoul ,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suk-Keun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, College of Dentistry, Kangreung National University , Kangneung ,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martijn","family":"Lolkema","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongbin","family":"Yim","sequence":"additional","affiliation":[{"name":"National Creative Research Initiative Center for Genetic Reprograming, Seoul National University , Seoul ,","place":["Korea"]},{"name":"Institute for Molecular Biology and Genetics, Seoul National University , Seoul ,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung H","family":"Hong","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biology and Genetics, Seoul National University , Seoul ,","place":["Korea"]},{"name":"Department of Molecular Biology, Seoul National University , Seoul ,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald H","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,7,1]]},"reference":[{"key":"2025032907443676700_R1","first-page":"445","volume-title":"Immunol. 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