{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:41:05Z","timestamp":1790829665290,"version":"4.1.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The final stage in the migration of leukocytes to sites of inflammation involves movement of leukocytes through the endothelial cell layer and the perivascular basement membrane. Both platelet-endothelial cell adhesion molecule-1 (PECAM-1\/CD31) and the integrin \u03b1v\u03b23 have been implicated in this process, and in vitro studies have identified \u03b1v\u03b23 as a heterotypic ligand for PECAM-1. In the present study we have addressed the roles of these molecules by investigating and comparing the effects of PECAM-1 and \u03b1v\u03b23 blockade on leukocyte migration in vivo. For this purpose we have examined the effects of neutralizing Abs directed against PECAM-1 (domain 1-specific, mAb 37) and \u03b23 integrins (mAbs 7E3 and F11) on leukocyte responses in the mesenteric microcirculation of anesthetized rats using intravital microscopy. The anti-PECAM-1 mAb suppressed leukocyte extravasation, but not leukocyte rolling or firm adhesion, elicited by IL-1\u03b2 in a dose-dependent manner (e.g., 67% inhibition at 10 mg\/kg 37 Fab), but had no effect on FMLP-induced leukocyte responses. Analysis by electron microscopy suggested that this suppression was due to an inhibition of neutrophil migration through the endothelial cell barrier. By contrast, both anti-\u03b23 integrin mAbs, 7E3 F(ab\u2032)2 (5 mg\/kg) and F11 F(ab\u2032)2 (5 mg\/kg), selectively reduced leukocyte extravasation induced by FMLP (38 and 46%, respectively), but neither mAb had an effect on IL-1\u03b2-induced leukocyte responses. These findings indicate roles for both PECAM-1 and \u03b23 integrins in leukocyte extravasation, but do not support the concept that these molecules act as counter-receptors in mediating leukocyte transmigration.<\/jats:p>","DOI":"10.4049\/jimmunol.165.1.426","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:07:57Z","timestamp":1398038877000},"page":"426-434","source":"Crossref","is-referenced-by-count":62,"title":["Divergent Effects of Platelet-Endothelial Cell Adhesion Molecule-1 and \u03b23 Integrin Blockade on Leukocyte Transmigration In Vivo"],"prefix":"10.1093","volume":"165","author":[{"given":"Richard D","family":"Thompson","sequence":"first","affiliation":[{"name":"BHF Cardiovascular Medicine Unit, Imperial College School of Medicine at the National Heart and Lung Institute, Hammersmith Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew W","family":"Wakelin","sequence":"additional","affiliation":[{"name":"BHF Cardiovascular Medicine Unit, Imperial College School of Medicine at the National Heart and Lung Institute, Hammersmith Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Y","family":"Larbi","sequence":"additional","affiliation":[{"name":"BHF Cardiovascular Medicine Unit, Imperial College School of Medicine at the National Heart and Lung Institute, Hammersmith Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Dewar","sequence":"additional","affiliation":[{"name":"Electron Microscopy Unit, Royal Brompton and Harefield National Health Service Trust , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Asimakopoulos","sequence":"additional","affiliation":[{"name":"BHF Cardiovascular Medicine Unit, Imperial College School of Medicine at the National Heart and Lung Institute, Hammersmith Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A","family":"Horton","sequence":"additional","affiliation":[{"name":"University College London Medical School , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian T","family":"Nakada","sequence":"additional","affiliation":[{"name":"Centocor , Malvern, PA, 19355"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sussan","family":"Nourshargh","sequence":"additional","affiliation":[{"name":"BHF Cardiovascular Medicine Unit, Imperial College School of Medicine at the National Heart and Lung Institute, Hammersmith Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,7,1]]},"reference":[{"key":"2025032907442799400_R1","first-page":"827","volume-title":"Annu. 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