{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:11:11Z","timestamp":1791155471064,"version":"4.1.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>IL-12 activates murine and human B cells, but little information is available as to the expression and function of IL-12R on human B lymphocytes. Here we show that the latter cells, freshly isolated from human tonsils, expressed the transcripts of both \u03b21 and \u03b22 chains of IL-12R and that \u03b22 chain mRNA was selectively increased (4- to 5-fold) by incubation with Staphylococcus aureus Cowan I bacteria or IL-12. B cell stimulation with IL-12 induced de novo expression of the transcripts of the two chains of IL-18R, i.e., IL-1 receptor-related protein and accessory protein-like. Functional studies showed that both IL-12 and IL-18 signaled to B cells through the NF-\u03baB pathway. In the case of IL-12, no involvement of STAT transcription factors, and in particular of STAT-4, was detected. c-rel and p50 were identified as the members of NF-\u03baB family involved in IL-12-mediated signal transduction to B cells. IL-12 and IL-18 synergized in the induction of IFN-\u03b3 production by tonsillar B cells, but not in the stimulation of B cell differentiation, although either cytokine promoted IgM secretion in culture supernatants. Finally, naive but not germinal center or memory, tonsillar B cells were identified as the exclusive IL-12 targets in terms of induction of NF-\u03baB activation and of IFN-\u03b3 production.<\/jats:p>","DOI":"10.4049\/jimmunol.165.12.6880","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T23:33:40Z","timestamp":1398036820000},"page":"6880-6888","source":"Crossref","is-referenced-by-count":87,"title":["Expression and Function of IL-12 and IL-18 Receptors on Human Tonsillar B Cells"],"prefix":"10.1093","volume":"165","author":[{"given":"Irma","family":"Airoldi","sequence":"first","affiliation":[{"name":"Laboratory of Oncology, G. Gaslini Institute , Genoa ,","place":["Italy"]},{"name":"Wistar Institute of Anatomy and Biology , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Gri","sequence":"additional","affiliation":[{"name":"Wistar Institute of Anatomy and Biology , Philadelphia, PA 19104"},{"name":"Division of Experimental Oncology, Istituto per lo Studio e la Cura dei tumori , Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason D","family":"Marshall","sequence":"additional","affiliation":[{"name":"Wistar Institute of Anatomy and Biology , Philadelphia, PA 19104"},{"name":"Dynavax Technologies , Berkeley, CA 94705"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Corcione","sequence":"additional","affiliation":[{"name":"Laboratory of Oncology, G. 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