{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:36:26Z","timestamp":1790840186694,"version":"4.1.0"},"reference-count":72,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We have used a recombinant, biotinylated form of the mouse NK cell inhibitory receptor, Ly49A, to visualize the expression of MHC class I (MHC-I) ligands on living lymphoid cells. A panel of murine strains, including MHC congenic lines, was examined. We detected binding of Ly49A to cells expressing H-2Dd, H-2Dk, and H-2Dp but not to those expressing other MHC molecules. Cells of the MHC-recombinant strain B10.PL (H-2u) not only bound Ly49A but also inhibited cytolysis by Ly49A+ effector cells, consistent with the correlation of in vitro binding and NK cell function. Binding of Ly49A to H-2Dd-bearing cells of different lymphoid tissues was proportional to the level of H-2Dd expression and was not related to the lineage of the cells examined. These binding results, interpreted in the context of amino acid sequence comparisons and the recently determined three-dimensional structure of the Ly49A\/H-2Dd complex, suggest a role for amino acid residues at the amino-terminal end of the \u03b11 helix of the MHC-I molecule for Ly49A interaction. This view is supported by a marked decrease in affinity of an H-2Dd mutant, I52 M, for Ly49A. Thus, allelic variation of MHC-I molecules controls measurable affinity for the NK inhibitory receptor Ly49A and explains differences in functional recognition in different mouse strains.<\/jats:p>","DOI":"10.4049\/jimmunol.165.12.6922","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T23:33:40Z","timestamp":1398036820000},"page":"6922-6932","source":"Crossref","is-referenced-by-count":20,"title":["Mapping the Ligand of the NK Inhibitory Receptor Ly49A on Living Cells"],"prefix":"10.1093","volume":"165","author":[{"given":"Doo Hyun","family":"Chung","sequence":"first","affiliation":[{"name":"Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kannan","family":"Natarajan","sequence":"additional","affiliation":[{"name":"Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa F","family":"Boyd","sequence":"additional","affiliation":[{"name":"Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301","family":"Tormo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Biotecnologi\u0301a, Consejo Superior de Investigaciones Cientificas, Campus de la Universidad Auto\u0301noma de Madrid , Madrid ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy A","family":"Mariuzza","sequence":"additional","affiliation":[{"name":"Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute , Rockville, MD 20850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne M","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Howard Hughes Medical Institute, Washington University School of Medicine , St. Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H","family":"Margulies","sequence":"additional","affiliation":[{"name":"Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,12,15]]},"reference":[{"key":"2025032908194495300_R1","first-page":"189","volume-title":"Annu. 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