{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:57:33Z","timestamp":1791205053366,"version":"4.1.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human V\u03b39V\u03b42 T cells are considered to play an important role in brucellosis, as this population is dramatically increased in peripheral blood of patients during the acute phase of the infection. This T lymphocyte population has been largely demonstrated to be activated by small m.w. nonpeptidic molecules from natural or synthetic origin. We recently identified a nonpeptidic fraction of Brucella suis that specifically activates human V\u03b39V\u03b42 T cells. Using a two-separate-chambers system, we showed that Brucella fraction, as well as isopentenyl pyrophosphate-activated V\u03b39V\u03b42 T cells, impaired the multiplication of B. suis in differentiated THP-1 cells through TNF-\u03b1 and IFN-\u03b3 release. In the present study, using circulating V\u03b39V\u03b42 T cells and autologous monocytes infected with B. suis, we provide evidence that 1) intramonocytic multiplication of B. suis is impaired by supernatants of activated V\u03b39V\u03b42 T cells in part via TNF-\u03b1 and IFN-\u03b3, this impairment occurring without host cell lysis; 2) unstimulated V\u03b39V\u03b42 T cells can impair intracellular bacterial multiplication after their activation by soluble factors released by infected monocytes; and 3) activated V\u03b39V\u03b42 T cells lyse Brucella-infected monocytes in a contact-dependent manner. Taken together, these results provide evidence that V\u03b39V\u03b42 T cells, in addition to being directly activated by soluble nonpeptidic molecules, can be stimulated to become highly cytotoxic in the specific presence of infected monocytes; moreover, they suggest how V\u03b39V\u03b42 T cells could be triggered and respond as antibacterial effector cells in the early stages of Brucella infection.<\/jats:p>","DOI":"10.4049\/jimmunol.165.12.7133","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T19:33:40Z","timestamp":1398022420000},"page":"7133-7139","source":"Crossref","is-referenced-by-count":43,"title":["V\u03b39V\u03b42 T Cells Impair Intracellular Multiplication of\n                    <i>Brucella suis<\/i>\n                    in Autologous Monocytes Through Soluble Factor Release and Contact-Dependent Cytotoxic Effect"],"prefix":"10.1093","volume":"165","author":[{"given":"Florence","family":"Ottones","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale Unite\u0301 431, Microbiologie et Pathologie Cellulaire Infectieuse, Universite\u0301 de Montpellier II , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Dornand","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale Unite\u0301 431, Microbiologie et Pathologie Cellulaire Infectieuse, Universite\u0301 de Montpellier II , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aroem","family":"Naroeni","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale Unite\u0301 431, Microbiologie et Pathologie Cellulaire Infectieuse, Universite\u0301 de Montpellier II , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Liautard","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale Unite\u0301 431, Microbiologie et Pathologie Cellulaire Infectieuse, Universite\u0301 de Montpellier II , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Favero","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale Unite\u0301 431, Microbiologie et Pathologie Cellulaire Infectieuse, Universite\u0301 de Montpellier II , Montpellier ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,12,15]]},"reference":[{"key":"2026020213132832000_R1","first-page":"340","volume-title":"Immunol. 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