{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:13:37Z","timestamp":1793236417141,"version":"4.5.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Crohn\u2019 s disease (CD) is a chronic intestinal inflammatory disorder characterized by aberrant mucosal Th1 cell activation and production of IL-12, the major Th1-driving factor. The T cell response to IL-12 is dependent on the expression of a specific receptor composed of two subunits, termed IL-12R\u03b21 and IL-12R\u03b22. The content of IL-12R\u03b22, as measured at the mRNA level, is crucial in regulating Th1 differentiation. In this study we therefore investigated IL-12R\u03b22 RNA transcripts in CD. IL-12R\u03b22 expression was increased in active CD as well as Helicobacter pylori (HP)-associated gastritis and Salmonella colitis compared with that in inactive CD, ulcerative colitis, noninflammatory controls, and celiac disease. In contrast, IL-12R\u03b21 transcripts were expressed at comparable levels in all samples. In CD, IL-12R\u03b22 expression strictly correlated with tyrosine phosphorylation of STAT4, a key component of the IL-12-dependent Th1 polarization. This was associated with a pronounced expression of IFN-\u03b3. Transcripts for IL-12\/p40 were detected in CD, HP-positive, and Salmonella colitis patients, but not in celiac disease, indicating that IL-12R\u03b22 up-regulation occurs only in IL-12-associated Th1 gastrointestinal diseases. Finally, we showed that stimulation of lamina propria mononuclear cells with IL-12 enhanced IL-12R\u03b22, suggesting that IL-12 regulates IL-12R\u03b22 expression in human gastrointestinal mucosa. The data show that the signaling pathway used by IL-12 to induce Th1 differentiation is increased at the site of disease in CD, further supporting the view that IL-12\/IL-12R signals contribute to the inflammatory response in this condition.<\/jats:p>","DOI":"10.4049\/jimmunol.165.12.7234","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T23:33:40Z","timestamp":1398036820000},"page":"7234-7239","source":"Crossref","is-referenced-by-count":108,"title":["Up-Regulation of the IL-12 Receptor \u03b22 Chain in Crohn\u2019s Disease"],"prefix":"10.1093","volume":"165","author":[{"given":"Tiziana","family":"Parrello","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universita\u0300 Magna Graecia di Catanzaro , Catanzaro ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Monteleone","sequence":"additional","affiliation":[{"name":"Department of Pediatric Gastroenterology, St. Bartholomew\u2019s and the Royal London School of Medicine and Dentistry , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Cucchiara","sequence":"additional","affiliation":[{"name":"Dipartimento di Pediatria, Universita\u0300 di Napoli Federico II , Naples ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Monteleone","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universita\u0300 Magna Graecia di Catanzaro , Catanzaro ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladislava","family":"Sebkova","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universita\u0300 Magna Graecia di Catanzaro , Catanzaro ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Doldo","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universita\u0300 Magna Graecia di Catanzaro , Catanzaro ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Luzza","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universita\u0300 Magna Graecia di Catanzaro , Catanzaro ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Pallone","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Interna, Universita\u0300 di Roma tor Vergata , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,12,15]]},"reference":[{"key":"2025032908204350100_R1","first-page":"182","volume-title":"Gastroenterology","year":"1998"},{"key":"2025032908204350100_R2","first-page":"1169","volume-title":"Gastroenterology","year":"1997"},{"key":"2025032908204350100_R3","first-page":"823","volume-title":"Am. 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