{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:07:26Z","timestamp":1790492846181,"version":"4.1.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Our findings using B cells from either wild-type, CD86-deficient, or \u03b22-adrenergic receptor (\u03b22AR)-deficient mice suggest three mechanisms by which the level of IgG1 and IgE production can be increased on a per cell basis. Trinitrophenyl-specific B cells enriched from unimmunized mouse spleens were pre-exposed to Ag and\/or the \u03b22AR ligand terbutaline for 24 h before being activated by either a \u03b22AR-negative Th2 cell clone or CD40 ligand\/Sf9 cells and IL-4 in the presence or absence of an anti-CD86 Ab. Data suggest that the first mechanism involves a B cell receptor (BCR)-dependent up-regulation of CD86 expression that, when CD86 is stimulated, increases the amount of IgG1 and IgE produced in comparison to unstimulated cells. The second mechanism involves a BCR- and \u03b22AR-dependent up-regulation of CD86 to a level higher than that induced by stimulation of either receptor alone that, when CD86 is stimulated, further increases the amount of IgG1 and IgE produced. The third mechanism is BCR-independent and involves a \u03b22AR-dependent increase in the ability of a B cell to respond to IL-4. Flow cytometric and limiting dilution analyses suggest that the increase in IgG1 and IgE occurs independently from the isotype switching event. These findings suggest that the BCR, the \u03b22AR, and CD86 are involved in regulating IL-4-dependent IgG1 and IgE production.<\/jats:p>","DOI":"10.4049\/jimmunol.165.2.680","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T03:02:33Z","timestamp":1398135753000},"page":"680-690","source":"Crossref","is-referenced-by-count":123,"title":["Stimulation of the B Cell Receptor, CD86 (B7-2), and the \u03b22-Adrenergic Receptor Intrinsically Modulates the Level of IgG1 and IgE Produced per B Cell"],"prefix":"10.1093","volume":"165","author":[{"given":"Deborah J","family":"Kasprowicz","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Loyola University Medical Center , Maywood, IL 60153"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam P","family":"Kohm","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center , Maywood, IL 60153"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T","family":"Berton","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Health Science Center , San Antonio, TX 78284"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrezj J","family":"Chruscinski","sequence":"additional","affiliation":[{"name":"Department of Medicine and Molecular and Cellular Physiology, Stanford University , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlene","family":"Sharpe","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women\u2019s Hospital , Boston MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia M","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Loyola University Medical Center , Maywood, IL 60153"},{"name":"Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center , Maywood, IL 60153"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,7,15]]},"reference":[{"key":"2025032907464683100_R1","first-page":"425","volume-title":"Nature","year":"1994"},{"key":"2025032907464683100_R2","first-page":"591","volume-title":"Annu. 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