{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T06:42:58Z","timestamp":1776062578337,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Protective immunity against Leishmania major generated by DNA encoding the LACK (Leishmania homologue of receptor for activated C kinase) Ag has been shown to be more durable than vaccination with LACK protein plus IL-12. One mechanism to account for this may be the selective ability of DNA vaccination to induce CD8+ IFN-\u03b3-producing T cells. In this regard, we previously reported that depletion of CD8+ T cells in LACK DNA-vaccinated mice abrogated protection when infectious challenge was done 2 wk postvaccination. In this study, we extend these findings to study the mechanism by which CD8+ T cells induced by LACK DNA vaccination mediate both short- and long-term protective immunity against L. major. Mice vaccinated with LACK DNA and depleted of CD8+ T cells at the time of vaccination or infection were unable to control infection when challenge was done 2 or 12 wk postvaccination. Remarkably, it was noted that depletion of CD8+ T cells in LACK DNA-vaccinated mice was associated with a striking decrease in the frequency of LACK-specific CD4+ IFN-\u03b3-producing T cells both before and after infection. Moreover, data are presented to suggest a mechanism by which CD8+ T cells exert this regulatory role. Taken together, these data provide additional insight into how Th1 cells are generated and sustained in vivo and suggest a potentially novel immunoregulatory role for CD8+ T cells following DNA vaccination.<\/jats:p>","DOI":"10.4049\/jimmunol.165.2.915","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T23:02:33Z","timestamp":1398121353000},"page":"915-924","source":"Crossref","is-referenced-by-count":115,"title":["Requirements for the Maintenance of Th1 Immunity In Vivo Following DNA Vaccination: A Potential Immunoregulatory Role for CD8+ T Cells"],"prefix":"10.1093","volume":"165","author":[{"given":"Sanjay","family":"Gurunathan","sequence":"first","affiliation":[{"name":"Clinical Immunology Section, Laboratory of Clinical Investigation, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Stobie","sequence":"additional","affiliation":[{"name":"Clinical Immunology Section, Laboratory of Clinical Investigation, National Institutes of Health , Bethesda, MD 20892"},{"name":"Howard Hughes Medical Institute-National Institutes of Health Medical Research Scholars , San Francisco, CA 94143"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calmin","family":"Prussin","sequence":"additional","affiliation":[{"name":"Laboratory of Allergic Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L","family":"Sacks","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, and Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Glaichenhaus","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie Mole\u0301culaire et Cellulaire, Centre National de la Recherche Scientifique (CNRS) , Valbonne ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah J","family":"Fowell","sequence":"additional","affiliation":[{"name":"University of California , San Francisco, CA 94143"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard M","family":"Locksley","sequence":"additional","affiliation":[{"name":"University of California , San Francisco, CA 94143"},{"name":"Howard Hughes Medical Institute , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John T","family":"Chang","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute-National Institutes of Health Medical Research Scholars , San Francisco, CA 94143"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-You","family":"Wu","sequence":"additional","affiliation":[{"name":"Clinical Immunology Section, Laboratory of Clinical Investigation, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A","family":"Seder","sequence":"additional","affiliation":[{"name":"Clinical Immunology Section, Laboratory of Clinical Investigation, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,7,15]]},"reference":[{"key":"2026020213065449100_R1","first-page":"617","volume-title":"Annu. 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