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Here, we report that in primary human B lymphocytes and in Ramos B cells B cell receptor (BCR) stimulation induces rapid and transient redistribution of a subset of engaged BCRs to lipid rafts and phosphorylation of raft-associated tyrosine kinase substrates. Cholesterol sequestration disrupted the lipid rafts, preventing BCR redistribution, but did not inhibit tyrosine kinase activation or phosphorylation of mitogen-activated protein kinase\/extracellular regulated kinase. However, raft disruption enhanced the release of calcium from intracellular stores, suggesting that rafts may sequester early signaling events that down-regulate calcium flux. Consistent with this, BCR stimulation induced rapid and transient translocation of the Src homology 2 domain-containing inositol phosphatase, SHIP, into lipid rafts.<\/jats:p>","DOI":"10.4049\/jimmunol.165.3.1220","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T17:16:28Z","timestamp":1398186988000},"page":"1220-1227","source":"Crossref","is-referenced-by-count":103,"title":["Transient Translocation of the B Cell Receptor and Src Homology 2 Domain-Containing Inositol Phosphatase to Lipid Rafts: Evidence Toward a Role in Calcium Regulation"],"prefix":"10.1093","volume":"165","author":[{"given":"Ryan J","family":"Petrie","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul P M","family":"Schnetkamp","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamala D","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Calgary , Calgary, Alberta ,","place":["Canada"]},{"name":"Department of Physiology and Biophysics, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjula","family":"Awasthi-Kalia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie P","family":"Deans","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Calgary , Calgary, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,8,1]]},"reference":[{"key":"2026020213071244400_R1","first-page":"296","volume-title":"Curr. 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