{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:50:08Z","timestamp":1770483008251,"version":"3.49.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infection with EBV provides a unique opportunity to follow the human CD8+ T cell response to a persistent, genetically stable agent from the primary phase, as seen in infectious mononucleosis (IM) patients, into long-term memory. This study focuses on the response to an immunodominant HLA-A2.01-restricted epitope, GLCTLVAML, from the EBV-lytic cycle Ag BMLF1. TCR analysis of the highly amplified primary response to this epitope revealed markedly oligoclonal receptor usage among in vitro-derived clones, with similar clonotypes dominant in all three IM patients studied. Direct staining of IM T cell preparations with the A2.01\/GLCTLVAML tetramer linked this oligoclonal epitope-specific response with appropriate V\u03b2 subset expansions in the patients\u2019 blood. These patients were studied again &amp;gt;2 years later, at which time TCR analysis of in vitro-reactivated clones suggested that rare clonotypes within the primary response had now come to dominate memory. Five additional A2.01-positive IM patients were studied prospectively for V\u03b2 subset representation within primary and memory epitope-specific populations as identified by tetramer staining. In each case, the primary response contained large V\u03b22, V\u03b216, or V\u03b222 components, and in three of five cases the originally dominant V\u03b2 was represented very poorly, if at all, in memory. We conclude 1) that an EBV epitope-specific primary response large enough to account for up to 10% CD8+ T cells in IM blood may nevertheless be dominated by just a few highly expanded clonotypes, and 2) that with persistent viral challenge such dominant T cell clonotypes may be lost and replaced by others in memory.<\/jats:p>","DOI":"10.4049\/jimmunol.165.9.4831","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:59:08Z","timestamp":1398034748000},"page":"4831-4841","source":"Crossref","is-referenced-by-count":87,"title":["Changing Patterns of Dominant TCR Usage with Maturation of an EBV-Specific Cytotoxic T Cell Response"],"prefix":"10.1093","volume":"165","author":[{"given":"Nicola E","family":"Annels","sequence":"first","affiliation":[{"name":"Cancer Research Campaign Institute for Cancer Studies and Medical Research Council Centre for Immune Regulation, University of Birmingham , Edgbaston, Birmingham ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret F C","family":"Callan","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, John Radcliffe Hospital , Oxford ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, John Radcliffe Hospital , Oxford ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan B","family":"Rickinson","sequence":"additional","affiliation":[{"name":"Cancer Research Campaign Institute for Cancer Studies and Medical Research Council Centre for Immune Regulation, University of Birmingham , Edgbaston, Birmingham ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,11,1]]},"reference":[{"key":"2026020213100432800_R1","first-page":"959","volume-title":"Cell","year":"1986"},{"key":"2026020213100432800_R2","first-page":"395","volume-title":"Nature","year":"1988"},{"key":"2026020213100432800_R3","first-page":"134","volume-title":"Nature","year":"1996"},{"key":"2026020213100432800_R4","first-page":"209","volume-title":"Science","year":"1996"},{"key":"2026020213100432800_R5","first-page":"620","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020213100432800_R6","first-page":"477","volume-title":"Curr. Opin. Immunol.","year":"1997"},{"key":"2026020213100432800_R7","first-page":"6013","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020213100432800_R8","first-page":"1269","volume-title":"J. Immunol.","year":"1980"},{"key":"2026020213100432800_R9","first-page":"198","volume-title":"J. Infect. Dis.","year":"1985"},{"key":"2026020213100432800_R10","first-page":"6103","volume-title":"J. Virol.","year":"1994"},{"key":"2026020213100432800_R11","first-page":"1993","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020213100432800_R12","first-page":"71","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020213100432800_R13","first-page":"463","volume-title":"Nature","year":"1994"},{"key":"2026020213100432800_R14","first-page":"906","volume-title":"Nat. Med.","year":"1996"},{"key":"2026020213100432800_R15","first-page":"177","volume-title":"Immunity","year":"1998"},{"key":"2026020213100432800_R16","first-page":"167","volume-title":"Immunity","year":"1998"},{"key":"2026020213100432800_R17","first-page":"1383","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020213100432800_R18","first-page":"1395","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020213100432800_R19","first-page":"54","volume-title":"Science","year":"1996"},{"key":"2026020213100432800_R20","first-page":"255","volume-title":"Nature","year":"1999"},{"key":"2026020213100432800_R21","first-page":"5764","volume-title":"J. Virol.","year":"1997"},{"key":"2026020213100432800_R22","first-page":"61","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020213100432800_R23","first-page":"9848","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1997"},{"key":"2026020213100432800_R24","first-page":"453","volume-title":"Nature","year":"1991"},{"key":"2026020213100432800_R25","first-page":"2199","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020213100432800_R26","first-page":"2397","volume-title":"Fields Virology","year":"1996"},{"key":"2026020213100432800_R27","first-page":"157","volume-title":"J. Exp. Med.","year":"1992"},{"key":"2026020213100432800_R28","first-page":"169","volume-title":"J. Exp. Med.","year":"1992"},{"key":"2026020213100432800_R29","first-page":"405","volume-title":"Annu. Rev. Immunol.","year":"1997"},{"key":"2026020213100432800_R30","first-page":"2335","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020213100432800_R31","first-page":"1815","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020213100432800_R32","first-page":"4382","volume-title":"Eur. J. Immunol.","year":"1998"},{"key":"2026020213100432800_R33","first-page":"1801","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020213100432800_R34","first-page":"1605","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020213100432800_R35","first-page":"1745","volume-title":"Int. Immunol.","year":"1997"},{"key":"2026020213100432800_R36","first-page":"83","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020213100432800_R37","first-page":"65","volume-title":"J. Immunol. Methods","year":"1997"},{"key":"2026020213100432800_R38","first-page":"455","volume-title":"Immunogenetics","year":"1995"},{"key":"2026020213100432800_R39","first-page":"1261","volume-title":"Science","year":"1992"},{"key":"2026020213100432800_R40","first-page":"2001","volume-title":"J. Immunol.","year":"2000"},{"key":"2026020213100432800_R41","first-page":"485","volume-title":"Immunity","year":"1999"},{"key":"2026020213100432800_R42","first-page":"701","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020213100432800_R43","first-page":"106","volume-title":"Science","year":"1995"},{"key":"2026020213100432800_R44","first-page":"8987","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1991"},{"key":"2026020213100432800_R45","first-page":"7569","volume-title":"J. Virol.","year":"1996"},{"key":"2026020213100432800_R46","first-page":"3913","volume-title":"J. Virol.","year":"1992"},{"key":"2026020213100432800_R47","first-page":"2527","volume-title":"J. Virol.","year":"1992"},{"key":"2026020213100432800_R48"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/165\/9\/4831\/62734163\/4831.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/165\/9\/4831\/62734163\/4831.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T18:15:54Z","timestamp":1770056154000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/165\/9\/4831\/8038674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11,1]]},"references-count":48,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2000,11,1]]},"published-print":{"date-parts":[[2000,11,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.165.9.4831","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2000,11]]},"published":{"date-parts":[[2000,11,1]]}}}