{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:43:37Z","timestamp":1790390617397,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Despite the near universal association of congenital heart block and maternal Abs to SSA\/Ro and SSB\/La, the intracellular location of these Ags has made it difficult to substantiate their involvement in pathogenicity. To define whether components of the SSA\/Ro-SSB\/La complex, which translocate during apoptosis, are indeed accessible to extracellular Abs, two approaches were taken: immunoprecipitation of surface biotinylated proteins and scanning electron microscopy. Human fetal cardiocytes from 16\u201324-wk abortuses were cultured and incubated with staurosporine to induce apoptosis. Surface biotinylated 48-kDa SSB\/La was reproducibly immunoprecipitated from apoptotic, but not nonapoptotic cardiocytes. Surface expression of SSA\/Ro and SSB\/La was further substantiated by scanning electron microscopy. Gold particles (following incubation with gold-labeled sera containing various specificities of anti-SSA\/Ro-SSB\/La Abs and murine mAb to SSB\/La and 60-kDa SSA\/Ro) were consistently observed on early and late apoptotic cardiocytes. No particles were seen after incubation with control antisera. To evaluate whether opsonized apoptotic cardiocytes promote inflammation, cells were cocultured with macrophages. Compared with nonapoptotic cardiocytes or apoptotic cardiocytes incubated with normal sera, apoptotic cardiocytes preincubated with affinity-purified Abs to SSB\/La, 52-kDa SSA\/Ro, or 60-kDa SSA\/Ro increased the secretion of TNF-\u03b1 from cocultured macrophages. In summary, apoptosis results in surface accessibility of all SSA\/Ro-SSB\/La Ags for recognition by circulating maternal Abs. It is speculated that in vivo such opsonized apoptotic cardiocytes promote an inflammatory response by resident macrophages with damage to surrounding conducting tissue.<\/jats:p>","DOI":"10.4049\/jimmunol.165.9.5345","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:59:08Z","timestamp":1398034748000},"page":"5345-5351","source":"Crossref","is-referenced-by-count":170,"title":["Anti-SSA\/Ro and Anti-SSB\/La Autoantibodies Bind the Surface of Apoptotic Fetal Cardiocytes and Promote Secretion of TNF-\u03b1 by Macrophages"],"prefix":"10.1093","volume":"165","author":[{"given":"Maria-Eugenia","family":"Miranda-Caru\u0301s","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Hospital for Joint Diseases, New York University School of Medicine , New York, NY 10003"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca Dinu","family":"Askanase","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Hospital for Joint Diseases, New York University School of Medicine , New York, NY 10003"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M","family":"Clancy","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Hospital for Joint Diseases, New York University School of Medicine , New York, NY 10003"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Di Donato","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Hospital for Joint Diseases, New York University School of Medicine , New York, NY 10003"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tseng-Ming","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biochemical and Materials Engineering, Stevens Institute of Technology , Hoboken, NJ 07030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R","family":"Libera","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biochemical and Materials Engineering, Stevens Institute of Technology , Hoboken, NJ 07030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward K L","family":"Chan","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill P","family":"Buyon","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Hospital for Joint Diseases, New York University School of Medicine , New York, NY 10003"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,11,1]]},"reference":[{"key":"2026020213103595100_R1","first-page":"337","volume-title":"Systemic Lupus Erythematosus","year":"1999"},{"key":"2026020213103595100_R2","first-page":"5","volume-title":"APMIS","year":"1996"},{"key":"2026020213103595100_R3","first-page":"544","volume-title":"Ann. Intern. Med.","year":"1994"},{"key":"2026020213103595100_R4","first-page":"120","volume-title":"J. Rheumatol.","year":"1991"},{"key":"2026020213103595100_R5","first-page":"1588","volume-title":"Arthritis Rheum.","year":"1993"},{"key":"2026020213103595100_R6","first-page":"66","volume-title":"Semin. Dermatol.","year":"1988"},{"key":"2026020213103595100_R7","first-page":"2335","volume-title":"Arthritis Rheum.","year":"1999"},{"key":"2026020213103595100_R8","first-page":"1658","volume-title":"J. Am. Coll. Cardiol.","year":"1998"},{"key":"2026020213103595100_R9","first-page":"1317","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020213103595100_R10","first-page":"5061","volume-title":"J. Immunol.","year":"1998"},{"key":"2026020213103595100_R11","first-page":"279","volume-title":"Systemic Lupus Erythematosus","year":"1999"},{"key":"2026020213103595100_R12","first-page":"2033","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020213103595100_R13","first-page":"103","volume-title":"New Biol.","year":"1991"},{"key":"2026020213103595100_R14","first-page":"1","volume-title":"Eur. J. Biochem.","year":"1996"},{"key":"2026020213103595100_R15","first-page":"209","volume-title":"J. Mol. Cell. Cardiol.","year":"1996"},{"key":"2026020213103595100_R16","first-page":"260","volume-title":"Pediatr. Res.","year":"1999"},{"key":"2026020213103595100_R17","first-page":"291","volume-title":"Arch. Gesamte Physiol. Menschen Tiere Pflu\u0308gers","year":"1895"},{"key":"2026020213103595100_R18","first-page":"5886","volume-title":"J. Immunol.","year":"1998"},{"key":"2026020213103595100_R19","first-page":"865","volume-title":"J. Clin. Invest.","year":"1989"},{"key":"2026020213103595100_R20","first-page":"133","volume-title":"Neurology","year":"1983"},{"key":"2026020213103595100_R21","first-page":"169","volume-title":"J. Pediatr.","year":"1990"},{"key":"2026020213103595100_R22","first-page":"209","volume-title":"Autoimmunity","year":"1993"},{"key":"2026020213103595100_R23","first-page":"16917","volume-title":"J. Biol. Chem.","year":"1999"},{"key":"2026020213103595100_R24","first-page":"315","volume-title":"Cell Biol. Int.","year":"1997"},{"key":"2026020213103595100_R25","first-page":"2231","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020213103595100_R26","first-page":"576","volume-title":"Tissue Antigens","year":"1999"},{"key":"2026020213103595100_R27"},{"key":"2026020213103595100_R28","first-page":"350","volume-title":"Nature","year":"1997"},{"key":"2026020213103595100_R29","first-page":"890","volume-title":"J. Clin. Invest.","year":"1998"},{"key":"2026020213103595100_R30","first-page":"205","volume-title":"Arthritis Rheum.","year":"1998"},{"key":"2026020213103595100_R31","first-page":"1717","volume-title":"Science","year":"1998"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/165\/9\/5345\/62733634\/5345.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/165\/9\/5345\/62733634\/5345.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T18:16:53Z","timestamp":1770056213000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/165\/9\/5345\/8038725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11,1]]},"references-count":31,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2000,11,1]]},"published-print":{"date-parts":[[2000,11,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.165.9.5345","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2000,11]]},"published":{"date-parts":[[2000,11,1]]}}}