{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:41:27Z","timestamp":1771627287396,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The beige\/nude\/xid\/human (bnx\/hu) model of human hematopoiesis provides a unique opportunity to study extrathymic human T lymphocyte development in an in vivo system. Purified human hematopoietic stem cells develop into mature T lymphocytes and immature progenitors in the bone marrow of athymic bnx mice. The human T cells are all TCR\u03b1\u03b2+ and display a restricted TCRV\u03b2 repertoire. In the current studies, we examined the effects of systemic human IL-7 (huIL-7) administration on the phenotype and the activation status of the bnx\/hu T cells. In the majority of the mice that did not have huIL-7 administration, a higher frequency of human CD3+\/CD8+ than CD3+\/CD4+ T cells developed in the bone marrow. This phenomenon is also frequently observed in human bone marrow transplant recipients. Extremely low levels of IL-2 were expressed by human CD3+ cells isolated from these mice, in response to PMA plus ionomycin and to CD3 and CD28 cross-linking. IL-4 was not expressed by cells exposed to either stimulus, demonstrating a profound inability of the bnx\/hu T cells to produce this cytokine. Systemic production of huIL-7 from engineered stromal cells transplanted into the mice increased the human CD4 to CD8 ratios, and increased the ratio of memory to naive CD4+ and CD8+ T cells. The human CD3+ cells recovered from mice that had systemic huIL-7 and equivalent numbers of CD3+\/CD4+ and CD3+\/CD8+ cells in the marrow were still unable to produce IL-4 in response to any condition tested, but were capable of normal levels of IL-2 production following stimulation.<\/jats:p>","DOI":"10.4049\/jimmunol.166.1.170","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:32:59Z","timestamp":1398029579000},"page":"170-181","source":"Crossref","is-referenced-by-count":26,"title":["IL-7 Enhances the Responsiveness of Human T Cells That Develop in the Bone Marrow of Athymic Mice"],"prefix":"10.1093","volume":"166","author":[{"given":"Eleanor C","family":"Tsark","sequence":"first","affiliation":[{"name":"Division of Research Immunology\/Bone Marrow Transplantation, Childrens Hospital of Los Angeles , Los Angeles, CA 90027"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mo A","family":"Dao","sequence":"additional","affiliation":[{"name":"Division of Research Immunology\/Bone Marrow Transplantation, Childrens Hospital of Los Angeles , Los Angeles, CA 90027"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuli","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Research Immunology\/Bone Marrow Transplantation, Childrens Hospital of Los Angeles , Los Angeles, CA 90027"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Division of Research Immunology\/Bone Marrow Transplantation, Childrens Hospital of Los Angeles , Los Angeles, CA 90027"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan A","family":"Nolta","sequence":"additional","affiliation":[{"name":"Division of Research Immunology\/Bone Marrow Transplantation, Childrens Hospital of Los Angeles , Los Angeles, CA 90027"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,1,1]]},"reference":[{"key":"2026020208072755600_R1","first-page":"61","volume-title":"Immunol. 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