{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T18:23:51Z","timestamp":1771611831452,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Persistent immunity against Leishmania infections in humans is mediated predominantly by CD4+ T cells of the Th1 phenotype. Herein we report the expression cloning of eight Leishmania Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins \u03b1- and \u03b2-tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins have been previously reported as T cell-stimulating Ags from Leishmania. \u03b2-tubulin-specific T cell clones generated against Leishmania major amastigotes responded to Leishmania-infected macrophages and dendritic cells. IFN-\u03b3 enzyme-linked immunospot analysis demonstrated the presence of T cells specific for several of these Ags in PBMC from self-healing cutaneous leishmaniasis patients infected with either Leishmania tropica or L. major. The responses elicited by Leishmania histone H2b were particularly striking in terms of frequency of histone-specific T cells in PBMC (1 T cell of 6000 PBMC) as well as the percentage of responding donors (86%, 6 of 7). Ags identified by T cells from immune donors might constitute potential vaccine candidates for leishmaniasis.<\/jats:p>","DOI":"10.4049\/jimmunol.166.1.498","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:32:59Z","timestamp":1398029579000},"page":"498-505","source":"Crossref","is-referenced-by-count":67,"title":["Identification and Characterization of T Cell-Stimulating Antigens from\n                    <i>Leishmania<\/i>\n                    by CD4 T Cell Expression Cloning"],"prefix":"10.1093","volume":"166","author":[{"given":"Peter","family":"Probst","sequence":"first","affiliation":[{"name":"Corixa Corporation , Seattle, WA 98104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Stromberg","sequence":"additional","affiliation":[{"name":"Corixa Corporation , Seattle, WA 98104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hashim W","family":"Ghalib","sequence":"additional","affiliation":[{"name":"College of Medicine, King Khalid University , Abha ,","place":["Saudi Arabia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Mozel","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute , Seattle, WA 98104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Badaro","sequence":"additional","affiliation":[{"name":"Federal University of Bahia , Salvador, Bahia ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven G","family":"Reed","sequence":"additional","affiliation":[{"name":"Corixa Corporation , Seattle, WA 98104"},{"name":"Infectious Disease Research Institute , Seattle, WA 98104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa , Ottawa, Ontario ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,1,1]]},"reference":[{"key":"2026020208114257000_R1","first-page":"1191","volume-title":"Lancet","year":"1999"},{"key":"2026020208114257000_R2","first-page":"1","volume-title":"APMIS (Suppl.)","year":"1997"},{"key":"2026020208114257000_R3","first-page":"1","volume-title":"Clin. 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