{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T02:51:54Z","timestamp":1770087114669,"version":"3.49.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2001,6,1]],"date-time":"2001-06-01T00:00:00Z","timestamp":991353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Previous studies have shown that the DM-deficient cell line, T2-I-Ab, is very inefficient at presenting toxic shock syndrome toxin 1 (TSST-1) to T cells, suggesting that I-Ab-associated peptides play an essential role in the presentation of this superantigen. Consistent with this, the loading of an I-Ab-binding peptide, staphylococcal enterotoxin B 121\u2013136, onto T2-I-Ab cells enhanced TSST-1 presentation &amp;gt;1000-fold. However, despite extensive screening, no other peptides have been identified that significantly promote TSST-1 presentation. In addition, the peptide effect on TSST-1 presentation has been demonstrated only in the context of the tumor cell line T2-I-Ab. Here we show that peptides that do not promote TSST-1 presentation can be converted into \u201cpromoting\u201d peptides by the progressive truncation of C-terminal residues. These studies result in the identification of two peptides derived from IgGV heavy chain and I-E\u03b1 proteins that are extremely strong promoters of TSST-1 presentation (47,500- and 12,000-fold, respectively). We have also developed a system to examine the role of MHC class II-associated peptides in superantigen presentation using splenic APC taken directly ex vivo. The data confirmed that the length of the MHC class II-bound peptide plays a critical role in the presentation of TSST-1 by splenic APC and showed that different subpopulations of APC are equally peptide dependent in TSST-1 presentation. Finally, we demonstrated that the presentation of staphylococcal enterotoxin A, like TSST-1, is peptide dependent, whereas staphylococcal enterotoxin B presentation is peptide independent.<\/jats:p>","DOI":"10.4049\/jimmunol.166.11.6514","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:55:28Z","timestamp":1398034528000},"page":"6514-6522","source":"Crossref","is-referenced-by-count":11,"title":["Identification of MHC Class II-Associated Peptides That Promote the Presentation of Toxic Shock Syndrome Toxin-1 to T Cells"],"prefix":"10.1093","volume":"166","author":[{"given":"Robert J","family":"Hogan","sequence":"first","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josine","family":"VanBeek","sequence":"additional","affiliation":[{"name":"Department of Immunology, St. Jude Children\u2019s Research Hospital , Memphis, TN 38105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana R","family":"Broussard","sequence":"additional","affiliation":[{"name":"Department of Immunology, St. Jude Children\u2019s Research Hospital , Memphis, TN 38105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherri L","family":"Surman","sequence":"additional","affiliation":[{"name":"Department of Immunology, St. Jude Children\u2019s Research Hospital , Memphis, TN 38105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L","family":"Woodland","sequence":"additional","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,6,1]]},"reference":[{"key":"2026020209175548600_R1","first-page":"1066","volume-title":"Science","year":"1990"},{"key":"2026020209175548600_R2","first-page":"1717","volume-title":"Life Sci.","year":"1995"},{"key":"2026020209175548600_R3","first-page":"435","volume-title":"Annu. Rev. Immunol.","year":"1999"},{"key":"2026020209175548600_R4","first-page":"807","volume-title":"Immunity","year":"1998"},{"key":"2026020209175548600_R5","first-page":"1083","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020209175548600_R6","first-page":"772","volume-title":"Eur. J. Immunol.","year":"1997"},{"key":"2026020209175548600_R7","first-page":"187","volume-title":"Immunity","year":"1995"},{"key":"2026020209175548600_R8","first-page":"2894","volume-title":"Eur. J. Immunol.","year":"1995"},{"key":"2026020209175548600_R9","first-page":"1870","volume-title":"Science","year":"1994"},{"key":"2026020209175548600_R10","first-page":"1211","volume-title":"Biochem. Biophys. Res. Commun.","year":"1993"},{"key":"2026020209175548600_R11","first-page":"596","volume-title":"Biochem. Biophys. Res. Commun.","year":"1994"},{"key":"2026020209175548600_R12","first-page":"531","volume-title":"Cell","year":"1996"},{"key":"2026020209175548600_R13","first-page":"543","volume-title":"Cell","year":"1996"},{"key":"2026020209175548600_R14","first-page":"254","volume-title":"Nature","year":"1987"},{"key":"2026020209175548600_R15","first-page":"5153","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209175548600_R16","first-page":"433","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020209175548600_R17","first-page":"4893","volume-title":"J. Immunol.","year":"1994"},{"key":"2026020209175548600_R18","first-page":"1931","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020209175548600_R19","first-page":"369","volume-title":"Int. Immunol.","year":"1994"},{"key":"2026020209175548600_R20","first-page":"964","volume-title":"Science","year":"1990"},{"key":"2026020209175548600_R21","first-page":"1198","volume-title":"J. Exp. Med.","year":"1981"},{"key":"2026020209175548600_R22","first-page":"868","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020209175548600_R23","first-page":"631","volume-title":"J. Exp. Med.","year":"1990"},{"key":"2026020209175548600_R24","first-page":"1693","volume-title":"J. Exp. Med.","year":"1992"},{"key":"2026020209175548600_R25","first-page":"18","volume-title":"Immunol. Today","year":"1997"},{"key":"2026020209175548600_R26","first-page":"622","volume-title":"Nature","year":"1991"},{"key":"2026020209175548600_R27","first-page":"429","volume-title":"Nature","year":"1992"},{"key":"2026020209175548600_R28","first-page":"163","volume-title":"Annu. Rev. Cell Biol.","year":"1993"},{"key":"2026020209175548600_R29","first-page":"187","volume-title":"Immunity","year":"1997"},{"key":"2026020209175548600_R30","first-page":"1278","volume-title":"Science","year":"1996"},{"key":"2026020209175548600_R31","first-page":"197","volume-title":"Immunity","year":"1997"},{"key":"2026020209175548600_R32","first-page":"3583","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020209175548600_R33","first-page":"3972","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1991"},{"key":"2026020209175548600_R34","first-page":"271","volume-title":"Curr. Opin. Immunol.","year":"1996"},{"key":"2026020209175548600_R35","first-page":"745","volume-title":"Annu. Rev. Immunol.","year":"1991"},{"key":"2026020209175548600_R36","first-page":"711","volume-title":"Nature","year":"1994"},{"key":"2026020209175548600_R37","first-page":"1874","volume-title":"Science","year":"1994"},{"key":"2026020209175548600_R38","first-page":"2605","volume-title":"Eur. J. Immunol.","year":"1998"},{"key":"2026020209175548600_R39","first-page":"1748","volume-title":"Eur. J. Immunol.","year":"2000"},{"key":"2026020209175548600_R40","first-page":"2583","volume-title":"J. Immunol.","year":"1989"},{"key":"2026020209175548600_R41","first-page":"1817","volume-title":"Science","year":"1992"},{"key":"2026020209175548600_R42","first-page":"764","volume-title":"Nature","year":"1992"},{"key":"2026020209175548600_R43","first-page":"27","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020209175548600_R44","first-page":"473","volume-title":"Immunity","year":"1999"},{"key":"2026020209175548600_R45","first-page":"6","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020209175548600_R46","first-page":"823","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020209175548600_R47","first-page":"2173","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020209175548600_R48","first-page":"1154","volume-title":"J. Immunol.","year":"1994"},{"key":"2026020209175548600_R49","first-page":"1079","volume-title":"Int. Immunol.","year":"1995"},{"key":"2026020209175548600_R50","first-page":"633","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020209175548600_R51","first-page":"87","volume-title":"J. Immunol.","year":"1993"},{"key":"2026020209175548600_R52","first-page":"42","volume-title":"Cell. Immunol.","year":"1998"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/166\/11\/6514\/62533192\/6514.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/166\/11\/6514\/62533192\/6514.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T14:25:03Z","timestamp":1770042303000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/166\/11\/6514\/8027035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6,1]]},"references-count":52,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2001,6,1]]},"published-print":{"date-parts":[[2001,6,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.166.11.6514","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2001,6]]},"published":{"date-parts":[[2001,6,1]]}}}