{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:33:40Z","timestamp":1791405220036,"version":"4.3.3"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have investigated the chemokine receptor expression and migratory behavior of a new subset of nickel-specific skin-homing regulatory CD4+ T cells (ThIL-10) releasing high levels of IL-10, low IFN-\u03b3, and undetectable IL-4. These cells inhibit in a IL-10-dependent manner the capacity of dendritic cells to activate nickel-specific Tc1 and Th1 lymphocytes. RNase protection assay and FACS analysis revealed the expression of a vast repertoire of chemokine receptors on resting ThIL-10, including the Th1-associated CXCR3 and CCR5, and the Th2-associated CCR3, CCR4, and CCR8, the latter at higher levels compared with Th2 cells. The most active chemokines for resting ThIL-10, in terms of calcium mobilization and in vitro migration, were in order of potency: CCL2 (monocyte chemoattractant protein-1, CCR2 ligand), CCL4 (macrophage-inflammatory protein-1\u03b2, CCR5 ligand), CCL3 (macrophage-inflammatory protein-1\u03b1, CCR1\/5 ligand), CCL17 (thymus and activation-regulated chemokine, CCR4 ligand), CCL1 (I-309, CCR8 ligand), CXCL12 (stromal-derived factor-1, CXCR4), and CCL11 (eotaxin, CCR3 ligand). Consistent with receptor expression down-regulation, activated ThIL-10 exhibited a reduced or absent response to most chemokines, but retained a significant migratory capacity to I-309, monocyte chemoattractant protein-1, and thymus and activation-regulated chemokine. I-309, which was ineffective on Th1 lymphocytes, attracted more efficiently ThIL-10 than Th2 cells. I-309 and CCR8 mRNAs were not detected in unaffected skin and were up-regulated at the skin site of nickel-allergic reaction, with an earlier expression kinetics compared with IL-10 and IL-4. Results indicate that skin-homing regulatory ThIL-10 lymphocytes coexpress functional Th1- and Th2-associated chemokine receptors, and that CCR8\/I-309-driven recruitment of both resting and activated ThIL-10 cells may be critically involved in the regulation of Th1-mediated skin allergic disorders.<\/jats:p>","DOI":"10.4049\/jimmunol.166.2.996","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:56:47Z","timestamp":1398034607000},"page":"996-1002","source":"Crossref","is-referenced-by-count":168,"title":["Chemokine Receptor Expression and Function in CD4+ T Lymphocytes with Regulatory Activity"],"prefix":"10.1093","volume":"166","author":[{"given":"Silvia","family":"Sebastiani","sequence":"first","affiliation":[{"name":"Laboratory of Immunology, Istituto Dermopatico dell\u2019Immacolata, IRCCS , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Allavena","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri , Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Albanesi","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Istituto Dermopatico dell\u2019Immacolata, IRCCS , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Nasorri","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Istituto Dermopatico dell\u2019Immacolata, IRCCS , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri , Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Traidl","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Istituto Dermopatico dell\u2019Immacolata, IRCCS , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvano","family":"Sozzani","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri , Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giampiero","family":"Girolomoni","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Istituto Dermopatico dell\u2019Immacolata, IRCCS , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Cavani","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Istituto Dermopatico dell\u2019Immacolata, IRCCS , Rome ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,1,15]]},"reference":[{"key":"2026020208123525700_R1","first-page":"872","volume-title":"Curr. 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