{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T01:32:52Z","timestamp":1770082372152,"version":"3.49.0"},"reference-count":65,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bronchial-alveolar eosinophilic inflammation is among the characteristic pathological changes in asthma, which has been shown to be correlated with type 2 cytokine and chemokine production. Exogenous IL-12 has been found to be inhibitory for pulmonary eosinophilia in reported studies. Using a murine asthma-like model induced by OVA, we found in the present study that IL-12 gene knockout (KO) mice showed substantially reduced airway recruitment of eosinophils compared with wild-type control mice following OVA sensitization\/challenge, although the levels of circulating eosinophils were comparable in these two groups of mice. Cytokine analysis showed Ag-driven Th1 (IFN-\u03b3) and Th2 (IL-4, IL-5, IL-10, and IL-13) cytokine production by CD4 T cells from local draining lymph nodes and spleen. Similarly, local eotaxin production was comparable in wild-type and IL-12 KO mice. In contrast, immunohistochemical analysis showed that the expression of VCAM-1 on the lung endothelium of IL-12 KO mice was dramatically less than that in wild-type mice. Furthermore, administration of rIL-12 at the stage of sensitization and challenge with OVA restored airway eosinophilia and VCAM-1 expression in IL-12 KO mice. The results suggest that endogenous IL-12 contributes to the recruitment of eosinophils into airways observed in asthma, possibly via enhancement of the expression of VCAM-1 on local vascular endothelial cells.<\/jats:p>","DOI":"10.4049\/jimmunol.166.4.2741","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:56:08Z","timestamp":1398034568000},"page":"2741-2749","source":"Crossref","is-referenced-by-count":35,"title":["IL-12-Dependent Vascular Cell Adhesion Molecule-1 Expression Contributes to Airway Eosinophilic Inflammation in a Mouse Model of Asthma-Like Reaction"],"prefix":"10.1093","volume":"166","author":[{"given":"Shuhe","family":"Wang","sequence":"first","affiliation":[{"name":"Immune Regulation of Allergy Research Group, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijun","family":"Fan","sequence":"additional","affiliation":[{"name":"Immune Regulation of Allergy Research Group, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaobing","family":"Han","sequence":"additional","affiliation":[{"name":"Immune Regulation of Allergy Research Group, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Immune Regulation of Allergy Research Group, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Bilenki","sequence":"additional","affiliation":[{"name":"Immune Regulation of Allergy Research Group, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Immune Regulation of Allergy Research Group, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,2,15]]},"reference":[{"key":"2026020209152174100_R1","first-page":"935","volume-title":"J. Allergy Clin. Immunol.","year":"1991"},{"key":"2026020209152174100_R2","first-page":"177","volume-title":"Adv. Immunol.","year":"1986"},{"key":"2026020209152174100_R3","first-page":"1117","volume-title":"J. Clin. Invest.","year":"1996"},{"key":"2026020209152174100_R4","first-page":"1033","volume-title":"N. Engl. J. Med.","year":"1990"},{"key":"2026020209152174100_R5","first-page":"1407","volume-title":"Am. Rev. Respir. Dis.","year":"1990"},{"key":"2026020209152174100_R6","first-page":"637","volume-title":"J Allergy Clin. Immunol.","year":"1991"},{"key":"2026020209152174100_R7","first-page":"211","volume-title":"Allergy","year":"1978"},{"key":"2026020209152174100_R8","first-page":"138","volume-title":"Immunol. Today.","year":"1996"},{"key":"2026020209152174100_R9","first-page":"787","volume-title":"Nature","year":"1996"},{"key":"2026020209152174100_R10","first-page":"263","volume-title":"Immunol. Today","year":"1997"},{"key":"2026020209152174100_R11","first-page":"835","volume-title":"Curr. Opin. Immunol.","year":"1994"},{"key":"2026020209152174100_R12","first-page":"298","volume-title":"N. Engl. J. Med.","year":"1992"},{"key":"2026020209152174100_R13","first-page":"881","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020209152174100_R14","first-page":"1","volume-title":"Immunity","year":"1996"},{"key":"2026020209152174100_R15","first-page":"604","volume-title":"J. Clin. Invest.","year":"1996"},{"key":"2026020209152174100_R16","first-page":"601","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020209152174100_R17","first-page":"785","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020209152174100_R18","first-page":"1657","volume-title":"J. Clin. Invest.","year":"1997"},{"key":"2026020209152174100_R19","first-page":"4593","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020209152174100_R20","first-page":"L219","volume-title":"Am. J. Physiol.","year":"1997"},{"key":"2026020209152174100_R21","first-page":"20","volume-title":"J. Clin. Invest.","year":"1991"},{"key":"2026020209152174100_R22","first-page":"3419","volume-title":"J. Immunol.","year":"1991"},{"key":"2026020209152174100_R23","first-page":"7430","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1991"},{"key":"2026020209152174100_R24","first-page":"1553","volume-title":"J. Exp. Med.","year":"1991"},{"key":"2026020209152174100_R25","first-page":"445","volume-title":"Am. J .Respir. Cell Mol. Biol.","year":"1991"},{"key":"2026020209152174100_R26","first-page":"1145","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020209152174100_R27","first-page":"2558","volume-title":"J. Immunol.","year":"1990"},{"key":"2026020209152174100_R28","first-page":"865","volume-title":"J. Immunol.","year":"1990"},{"key":"2026020209152174100_R29"},{"key":"2026020209152174100_R30","first-page":"730","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209152174100_R31","first-page":"5054","volume-title":"J. Immunol.","year":"1998"},{"key":"2026020209152174100_R32","first-page":"471","volume-title":"Immunity","year":"1996"},{"key":"2026020209152174100_R33","first-page":"1527","volume-title":"J. Exp. Med.","year":"1995"},{"key":"2026020209152174100_R34","first-page":"4173","volume-title":"J. Immunol.","year":"1996"},{"key":"2026020209152174100_R35","first-page":"317","volume-title":"Am. J. Respir. Cell Mol. Biol.","year":"1999"},{"key":"2026020209152174100_R36","first-page":"413","volume-title":"Eur. J. Immunol.","year":"1998"},{"key":"2026020209152174100_R37","first-page":"329","volume-title":"Immunology","year":"1999"},{"key":"2026020209152174100_R38","first-page":"382","volume-title":"Eur. J. Immunol.","year":"2000"},{"key":"2026020209152174100_R39","first-page":"2954","volume-title":"J. Immunol.","year":"1992"},{"key":"2026020209152174100_R40","first-page":"349","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020209152174100_R41","first-page":"74","volume-title":"Immunology","year":"1993"},{"key":"2026020209152174100_R42","first-page":"495","volume-title":"Annu. Rev. Immunol.","year":"1998"},{"key":"2026020209152174100_R43","first-page":"29","volume-title":"Annu. Rev. Immunol.","year":"1993"},{"key":"2026020209152174100_R44","first-page":"10188","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1993"},{"key":"2026020209152174100_R45","first-page":"1132","volume-title":"J. Immunol.","year":"1998"},{"key":"2026020209152174100_R46","first-page":"827","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020209152174100_R47","first-page":"1462","volume-title":"Blood.","year":"1991"},{"key":"2026020209152174100_R48","first-page":"549","volume-title":"Immunol. Ser.","year":"1992"},{"key":"2026020209152174100_R49","first-page":"308","volume-title":"Science","year":"1989"},{"key":"2026020209152174100_R50","first-page":"15","volume-title":"Immunity","year":"1996"},{"key":"2026020209152174100_R51","first-page":"195","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020209152174100_R52","first-page":"457","volume-title":"Am J Pathol.","year":"1998"},{"key":"2026020209152174100_R53","first-page":"1531","volume-title":"Cancer Res.","year":"1999"},{"key":"2026020209152174100_R54","first-page":"4481","volume-title":"J .Immunol.","year":"2000"},{"key":"2026020209152174100_R55","first-page":"35","volume-title":"Am. J. Respir. Cell Mol. Biol.","year":"1993"},{"key":"2026020209152174100_R56","first-page":"643","volume-title":"Cell","year":"1996"},{"key":"2026020209152174100_R57","first-page":"264","volume-title":"J. Clin. Invest.","year":"1995"},{"key":"2026020209152174100_R58","first-page":"1737","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020209152174100_R59","first-page":"1831","volume-title":"Eur. J. Immunol.","year":"1993"},{"key":"2026020209152174100_R60","first-page":"200","volume-title":"Immunol. Today","year":"1999"},{"key":"2026020209152174100_R61","first-page":"169","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020209152174100_R62","first-page":"6230","volume-title":"EMBO J.","year":"1998"},{"key":"2026020209152174100_R63","first-page":"9305","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1999"},{"key":"2026020209152174100_R64","first-page":"767","volume-title":"Annu. Rev. Immunol.","year":"2000"},{"key":"2026020209152174100_R65","first-page":"347","volume-title":"Eur. J. Immunol.","year":"2000"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/166\/4\/2741\/62535773\/2741.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/166\/4\/2741\/62535773\/2741.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T14:20:59Z","timestamp":1770042059000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/166\/4\/2741\/8025885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":65,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2001,2,15]]},"published-print":{"date-parts":[[2001,2,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.166.4.2741","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2001,2]]},"published":{"date-parts":[[2001,2]]}}}